Proceedings of the 2024 10th International Conference on Advances in Energy Resources and Environment Engineering (ICAESEE 2024)

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An Innovative IAC-HPLC-FLD Internal Standard Method Based on AFB1 Structural Modifications

Authors
Wenjing Liu1, Jiaying Zhao1, Renrong Liu1, *
1School of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi Province, 330013, China
*Corresponding author. Email: 694700017@qq.com
Corresponding Author
Renrong Liu
Available Online 9 May 2025.
DOI
10.2991/978-94-6463-708-3_37How to use a DOI?
Keywords
AFB1; Immunoaffinity Column; High-Performance Liquid Chromatography (HPLC); Internal Standard Method
Abstract

An innovative immunoaffinity column-based high-performance liquid chromatography (IAC-HPLC) approach was established, incorporating the synthesized structural analog AFB1-OH as an internal standard for the quantitative analysis of aflatoxin B1 (AFB1). AFB1-OH was synthesized via oximation and employed to quantify AFB1 levels in grain samples collected from different regions. Grain samples were extracted with a methanol-ultrapure water mixture (7:3, v/v), purified using IAC, and analyzed via HPLC with internal standard quantification. The method demonstrated outstanding linearity across a concentration range of 0.0625–50 ng/mL, achieving a correlation coefficient (R2) greater than 0.9998. The detection limit (LOD) and quantification limit (LOQ) were established at 0.55 ng/mL and 1.29 ng/mL, respectively. Recovery experiments indicated that the average recovery rates of AFB1 ranged between 95.09% and 106.54%, with variation coefficients (CVs) maintained below 5.76%. These findings highlight that AFB1-OH exhibits excellent stability and cost efficiency, while the developed method offers simplicity, high sensitivity, accuracy, and precision. This robust approach provides a reliable solution for determining AFB1 in diverse grain samples.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2024 10th International Conference on Advances in Energy Resources and Environment Engineering (ICAESEE 2024)
Series
Atlantis Highlights in Engineering
Publication Date
9 May 2025
ISBN
978-94-6463-708-3
ISSN
2589-4943
DOI
10.2991/978-94-6463-708-3_37How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Wenjing Liu
AU  - Jiaying Zhao
AU  - Renrong Liu
PY  - 2025
DA  - 2025/05/09
TI  - An Innovative IAC-HPLC-FLD Internal Standard Method Based on AFB1 Structural Modifications
BT  - Proceedings of the 2024 10th International Conference on Advances in Energy Resources and Environment Engineering (ICAESEE 2024)
PB  - Atlantis Press
SP  - 340
EP  - 346
SN  - 2589-4943
UR  - https://doi.org/10.2991/978-94-6463-708-3_37
DO  - 10.2991/978-94-6463-708-3_37
ID  - Liu2025
ER  -