Study of Morphology and Protein Crystal Character of Bacillus thuringiensis Isolates

Kusuma Handayani; Rachmat Nugraha Indra; Rochmah Agustrina; Sumardi

doi:10.2991/978-94-6463-730-4_24

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Study of Morphology and Protein Crystal Character of Bacillus thuringiensis Isolates

Authors

Kusuma Handayani¹^{, *}, Rachmat Nugraha Indra¹, Rochmah Agustrina¹, Sumardi¹

¹Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University, Jl. Prof. Sumantri Brojonegoro No.1, Bandar Lampung, 35145, Indonesia

^*Corresponding author. Email: kusuma.handayani@fmipa.unila.ac.id

Corresponding Author

Kusuma Handayani

Available Online 27 May 2025.

DOI: 10.2991/978-94-6463-730-4_24 How to use a DOI?
Keywords: Bacillus thuringiensis; Crystal Protein; Morphology
Abstract: Bacillus thuringiensis (Bt) has characteristics that it can produce protein crystals that are toxic to insects. The crystals consist of crystalline (Cry) and cytolytic (Cyt) proteins, known as δ-endotoxins. The specificity and activity of Bt on the substance are associated with the type and form of the δ-endotoxin. This research was conducted to determine the initial formation of protein crystals and to measure the number of bacterial cells during their growth and to characterize the shape of protein crystals in Bt isolates from the soil of the Liwa Botanical Garden Lampung. Forming Bt bacteria through protein crystal morphology can be done by Scanning Electron Microscope. The results showed that each isolate had more than one protein crystal shape with seven different variations, namely ovoid, spherical, triangular bipyramidal, rectangle, elongate, cuboid, and geometrical. By observing the time of formation, protein crystals were seen at the 3rd to 18th hour of growth.
Copyright: © 2025 The Author(s)
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 5th International Conference on Applied Sciences, Mathematics, and Informatics (ICASMI 2024)
Series: Advances in Physics Research
Publication Date: 27 May 2025
ISBN: 978-94-6463-730-4
ISSN: 2352-541X
DOI: 10.2991/978-94-6463-730-4_24 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Kusuma Handayani
AU  - Rachmat Nugraha Indra
AU  - Rochmah Agustrina
AU  - Sumardi
PY  - 2025
DA  - 2025/05/27
TI  - Study of Morphology and Protein Crystal Character of Bacillus thuringiensis Isolates
BT  - Proceedings of the 5th International Conference on Applied Sciences, Mathematics, and Informatics (ICASMI 2024)
PB  - Atlantis Press
SP  - 280
EP  - 293
SN  - 2352-541X
UR  - https://doi.org/10.2991/978-94-6463-730-4_24
DO  - 10.2991/978-94-6463-730-4_24
ID  - Handayani2025
ER  -

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