Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)

Green Grape Seed Extract-Mediated Synthesis of CuO Nanoparticles: Characterization and Enhanced Antibacterial Activity

Authors
V. Senthilkumar1, K. K. Ilavenil2, *, A. Kasthuri3, P. Pandian4
1Department of Mechanical Engineering, SRM TRP Engineering College, Tiruchirappalli, 621105, Tamilnadu, India
2Department of Chemistry, School of Engineering and Technology, Dhanalakshmi Srinivasan University, Samayapuram, Tiruchirappalli, 621112, Tamilnadu, India
3Department of Chemistry, Nehru Memorial College (Autonomous)/Affiliated to Bharathidasan University, Puthanampatti, Tiruchirappalli, 621007, Tamilnadu, India
4Department of Chemistry, Thanthai Periyar Government Arts and Science College (Autonomous)/Affiliated to Bharathidasan University, Tiruchirappalli, 620023, Tamilnadu, India
*Corresponding author. Email: ilavenilkk.set@dsuniversity.ac.in
Corresponding Author
K. K. Ilavenil
Available Online 7 February 2025.
DOI
10.2991/978-94-6463-648-2_3How to use a DOI?
Keywords
Grape Seed Extract; Antimicrobial Activity; Functional Groups; Spectroscopic Analysis; Bioactive Agents
Abstract

Green grape seed extract was used to create copper oxide (CuO) nanoparticles as an environmentally acceptable stabilising and reducing agent. When copper sulphate pentahydrate was reduced with sodium hydroxide (NaOH), the colour changed from blue to greenish-black, indicating that the pH had been corrected to 9. Functional groups including O-H stretching and metal-oxygen bonding were validated by FTIR analysis, confirming the creation of nanoparticles. Absorbance peaks at 372.75 nm and 390.05 nm were visible in UV-Vis spectroscopy, emphasising electronic transitions and charge transfer. X-ray diffraction (XRD) verified a monoclinic crystal structure with a crystallite size of 30.55 nm, while dynamic light scattering (DLS) showed an average particle size of 181.6 nm with a Polydispersity Index of 0.185. The CuO nanoparticles exhibited notable antibacterial activity, exhibiting 40 mm, 42 mm, and 43 mm inhibitory zones.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
Series
Advances in Biological Sciences Research
Publication Date
7 February 2025
ISBN
978-94-6463-648-2
ISSN
2468-5747
DOI
10.2991/978-94-6463-648-2_3How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - V. Senthilkumar
AU  - K. K. Ilavenil
AU  - A. Kasthuri
AU  - P. Pandian
PY  - 2025
DA  - 2025/02/07
TI  - Green Grape Seed Extract-Mediated Synthesis of CuO Nanoparticles: Characterization and Enhanced Antibacterial Activity
BT  - Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
PB  - Atlantis Press
SP  - 21
EP  - 37
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-648-2_3
DO  - 10.2991/978-94-6463-648-2_3
ID  - Senthilkumar2025
ER  -