Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)

Characterization of chlorpyrifos-degrading bacteria from paddy field soil for bioremediation

Authors
R. Thiagu1, G. Abirami1, Neha Chitta1, K. Ashok Kumar1, M. Jayanthi1, Kumar Krishnan2, P. Senthilkumar3, M. Suganthi1, *
1Department of Biotechnology, Vels Institute of Science, Technology & Advanced Studies (VISTAS), Pallavaram, Chennai, Tamilnadu, 600117, India
2Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Nilai, 71800, Negeri Sembilan, Malaysia
3Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamilnadu, 603203, India
*Corresponding author. Email: sugenthi88@gmail.com
Corresponding Author
M. Suganthi
Available Online 7 February 2025.
DOI
10.2991/978-94-6463-648-2_9How to use a DOI?
Keywords
agricultural soil; bioremediation; chlorpyrifos; environmental pollution; organophosphate; 16S rRNA gene sequencing
Abstract

Chlorpyrifos a widely used organophosphate pesticide poses significant environmental hazards due to its persistence, toxicity and efficiency in contaminating soil and water resources. The urgent need for effective remediation strategies has led to increased interest in microbial bioremediation which utilizes the natural ability of microorganisms to degrade environmental pollutants. This study aimed to isolate and characterize chlorpyrifos degrading bacteria from the paddy field soil. Soil sample was collected from chlorpyrifos-treated fields and subjected to enrichment culture technique. The resulting bacterial isolates were screened for their degradation capabilities to assess their ability to metabolize chlorpyrifos. Based on the pesticide degradation potential, one isolate, PDB-1 was selected for further molecular characterization using 16S rRNA gene sequencing. This analysis revealed that among the chlorpyrifos-degrading bacterial community, Acinetobacter baumannii demonstrated significant pesticide degradation activity. The findings of this research enhance our understanding of microbial pathways involved in chlorpyrifos degradation and contribute to the development of sustainable bioremediation strategies for contaminated agricultural environments.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
Series
Advances in Biological Sciences Research
Publication Date
7 February 2025
ISBN
978-94-6463-648-2
ISSN
2468-5747
DOI
10.2991/978-94-6463-648-2_9How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - R. Thiagu
AU  - G. Abirami
AU  - Neha Chitta
AU  - K. Ashok Kumar
AU  - M. Jayanthi
AU  - Kumar Krishnan
AU  - P. Senthilkumar
AU  - M. Suganthi
PY  - 2025
DA  - 2025/02/07
TI  - Characterization of chlorpyrifos-degrading bacteria from paddy field soil for bioremediation
BT  - Proceedings of the International Conference on Bio-Based Environment for Sustainable Territory (ICBEST 2024)
PB  - Atlantis Press
SP  - 100
EP  - 110
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-648-2_9
DO  - 10.2991/978-94-6463-648-2_9
ID  - Thiagu2025
ER  -