Proceedings of the 2nd International Conference on Sciences, Mathematics, and Education 2023 (ICOSMED 2023)

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Genetic Variation of Santigi (Pemphis acidula J.R.Forst. & G.Forst.) based on ISSR Markers

Authors
Magfirahtul Jannah1, *, Dewi Wahyuni K. Baderan1, Sukirman Rahim2, Melisnawati H. Angio3
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Gorontalo, 96554, Bone Bolango, Indonesia
2Wallacea Research Center for Biodiversity Conservation and Climate Change, UniversitasNegeri Gorontalo, 96128, Gorontalo City, Indonesia
3Research Center for Plant Conservation and Botanic Gardens and Forestry, National Research and Innovation Agency, 16122, Bogor, Indonesia
*Corresponding author. Email: magfirahtuljannah@ung.ac.id
Corresponding Author
Magfirahtul Jannah
Available Online 28 July 2025.
DOI
10.2991/978-2-38476-410-5_19How to use a DOI?
Keywords
genetic variation; Pemphis acidula; ISSR makers
Abstract

Pemphis acidula J.R. Forst. & G. Forst. is a mangroveassociated plant that grows wild in rocky or sandy coastal areas. Despite being widely exploited due to its high economic value, no genetic diversity study of P. acidula has been conducted to date. This study assessed the genetic variation among P. acidula populations from three different geographical locations on the southern coast of Gorontalo Province, Sulawesi Island, using ten inter-simple sequence repeat (ISSR) markers. DNA was extracted from leaf samples and used for PCR assays. The presence and absence of bands generated from ISSR analysis were scored. A total of 124 amplicons were generated within a size range of 175–4500 bp, of which 110 were polymorphic. The ISSR analysis of P. acidula showed a high polymorphism rate of 88.92%, with a mean polymorphic information content (PIC) value of 0.49, indicating that the ten primers used can effectively assess the genetic diversity of P. acidula. Cluster analysis using UPGMA revealed a low similarity percentage of 22.1%, suggesting that the genetic variation of P. acidula in Gorontalo is high.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2nd International Conference on Sciences, Mathematics, and Education 2023 (ICOSMED 2023)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
28 July 2025
ISBN
978-2-38476-410-5
ISSN
2352-5398
DOI
10.2991/978-2-38476-410-5_19How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Magfirahtul Jannah
AU  - Dewi Wahyuni K. Baderan
AU  - Sukirman Rahim
AU  - Melisnawati H. Angio
PY  - 2025
DA  - 2025/07/28
TI  - Genetic Variation of Santigi (Pemphis acidula J.R.Forst. & G.Forst.) based on ISSR Markers
BT  - Proceedings of the 2nd International Conference on Sciences, Mathematics, and Education 2023 (ICOSMED 2023)
PB  - Atlantis Press
SP  - 199
EP  - 207
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-410-5_19
DO  - 10.2991/978-2-38476-410-5_19
ID  - Jannah2025
ER  -