Development of Genome Editing Technology and Its Applications to Plants

DOI

10.2991/978-94-6463-910-0_11How to use a DOI?

Keywords

genome editing; CRISPR-Cas9; CRISPR derivation system; disease-resisting plant breeding; nucleic acid test

Abstract

As a core tool of modern life science and agricultural biological technology, genome editing technology has evolved for three generations from zinc finger nucleases (ZFNs) and transcription activator-like effector nuclease (TALEN) to CRISPR-Cas system, which has improved the editing accuracy, efficiency and applicability significantly. In this study, the principle mechanism, technological characteristics of three generations of gene editing tools as well as their typical applications to medical treatment and plant breeding were reviewed systematically. Furthermore, the working principle and unique applications of new CRISPR derivation systems (Cas12 and Cas13) were discussed and its potentials in polygenic editing, RNA regulation and high-sensitivity molecular detection were exhibited. Through comparison of differences of various edition systems in targeting mechanism, editing objects and application scenes, this study emphasizes on the important values of CRISPR family tools in functional expansion and platform building. Moreover, research results provide theoretical foundation and technological references for the transferred applications of gene editing in accurate medicine and sustainable agricultural development.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ruisi Liu
PY  - 2025
DA  - 2025/12/15
TI  - Development of Genome Editing Technology and Its Applications to Plants
BT  - Proceedings of the 2025 2nd International Symposium on Agricultural Engineering and Biology (ISAEB 2025)
PB  - Atlantis Press
SP  - 96
EP  - 104
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-910-0_11
DO  - 10.2991/978-94-6463-910-0_11
ID  - Liu2025
ER  -