High Performance Liquid Chromatographic Optimization Study of Environmental Estrogens

DOI

10.2991/itms-15.2015.271How to use a DOI?

Keywords

HPLC, estrogen, milk

Abstract

Nine kinds of environmental estrogens including -estradiol, estrone, mestranol, ethisterone, ethinyloestradiol, diethylstilbestrol, cortisone, estriol and hydrocortisone were determined using high performance liquid chromatography (HPLC). The determination conditions were optimized that ProntSIL 120-5-C18-ace-EPS column as stationary phase, methanol/water (82.5/17.5, v/v) as mobile phase and flow rate as 1.0 mL/min. These compounds could be base-line separated in 15 minutes. The commercially available milk spiked with the estrogens was performed as the real sample. The recovery rate was in the range of 92.2%-103.7% while the relative standard deviation (RSD) was less than 10%.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jingyao Shen
AU  - Min Xue
PY  - 2015/11
DA  - 2015/11
TI  - High Performance Liquid Chromatographic Optimization Study of Environmental Estrogens
BT  - Proceedings of the 2015 International Conference on Industrial Technology and Management Science
PB  - Atlantis Press
SP  - 1111
EP  - 1114
SN  - 2352-538X
UR  - https://doi.org/10.2991/itms-15.2015.271
DO  - 10.2991/itms-15.2015.271
ID  - Shen2015/11
ER  -