Dual-Assay Antioxidant Profiling (DPPH and ABTS) and Phytochemical Screening of Scaevola taccada (Gaertn.) Roxb.: Establishing a Bioactive Baseline for Ocular Nanocarrier Development

DOI

10.2991/978-94-6239-836-8_7How to use a DOI?

Keywords

Scaevola taccada; DPPH; ABTS; antioxidant activity; phytochemical screening; cataract; nanostructured lipid carrier

Abstract

Cataract remains the leading cause of avoidable blindness worldwide, and and oxidative stress is central to its pathogenesis, yet no effective pharmacological adjuvant exists. Scaevola taccada (Gaertn.) Roxb., an Indo-Pacific coastal shrub, is a promising but underexplored antioxidant source whose reported activity rests almost exclusively on the DPPH assay. This study screened the secondary metabolite profile of its ethanolic fruit extract, determined and compared its radical-scavenging capacity by DPPH and ABTS, and established a bioactive baseline for subsequent nanostructured lipid carrier development. Fruits collected from Serdang Bedagai, North Sumatra, were macerated in 96% ethanol, yielding 10.0 ± 0.5% (w/w) of a viscous dark greenish-brown extract. Qualitative screening detected tannins, flavonoids, alkaloids, and saponins, whereas steroids and triterpenoids were not detected. The extract produced concentration-dependent DPPH inhibition (27.93–88.93% over 10–40 µg/mL; y = 2.0075x + 10.252, R² = 0.9905), giving an IC₅₀ of 19.79 µg/mL, approaching ascorbic acid assayed in parallel (11.72 µg/mL) and classified as very strong. In the ABTS system, inhibition ranged from 23.37 to 90.22% over 50–400 µg/mL (R² = 0.9649), giving an IC₅₀ of 188.48 µg/mL, classified as weak and diverging 9.52-fold from the DPPH value. This divergence is attributed to the slow hydrogen-atom-transfer kinetics of condensed tannins within the shorter ABTS reading window rather than to a genuine difference in capacity. The extract represents a promising antioxidant baseline for ocular nanocarrier development, and dual-assay reporting is essential to avoid method-dependent misclassification.

Copyright

© 2026 The Author(s)

Open Access

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TY  - CONF
AU  - Humairah Medina Liza Lubis
AU  - Salsabila Litahayu Maheswari
AU  - Pierre Adam Fanromi
AU  - Ahmad Jamil
AU  - Ellya Syahputri Lubis
AU  - Siti Gusti Nasywa
PY  - 2026
DA  - 2026/11/11
TI  - Dual-Assay Antioxidant Profiling (DPPH and ABTS) and Phytochemical Screening of Scaevola taccada (Gaertn.) Roxb.: Establishing a Bioactive Baseline for Ocular Nanocarrier Development
BT  - Proceedings of the3rd Malikussaleh International Conference on Health and Disaster Medicine 2026  (MICOHEDMED 2026)
PB  - Atlantis Press
SP  - 58
EP  - 69
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6239-836-8_7
DO  - 10.2991/978-94-6239-836-8_7
ID  - Lubis2026
ER  -