Proceedings of the 6th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BIOMIC 2025)

6th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BIOMIC 2025)

📍Yogyakarta, Indonesia🗓️ 30-31 July 2025

Pig DNA Extraction Using the Ultrasound Method for Halal Product Authentication

Authors
Pramuja Baydillah1, Ulfah Lailatul Khoiriah1, Livya Safira Islam1, Fadlilatul Taufany1, Nurul Jadid2, Andi Yudha Sasongko3, Setiyo Gunawan1, 4, *
1Chemical Engineering Department, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
2Biology Department, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
3PT Biotek Prima Indoplus, Sidoarjo, Indonesia
4Halal Study Center, Institut Teknologi Sepuluh Nopember, Surabaya, Indonesia
*Corresponding author. Email: setiyo.gunawan@its.ac.id
Corresponding Author
Setiyo Gunawan
Available Online 4 September 2026.
DOI
10.2991/978-94-6239-762-0_17How to use a DOI?
Keywords
Cosmetics; Deoxyribonucleic Acid Extraction; Medicine; Processed Food; Ultrasound
Abstract

Deoxyribonucleic acid (DNA) extraction is the process of isolating DNA from cells or tissues of living organisms for further analysis. DNA extraction must be performed in matrix analysis of a sample to identify nucleic acids. This study evaluated the effectiveness of ultrasound in extracting pig DNA, with variations in sample type, power, and extraction time as independent variables. The results of the study showed the highest crude DNA pellets in the three samples (processed food, medicine, and cosmetics), which were 40.20 ± 1.10 ng, 0.69 ± 0.18 ng, and 0.10 ± 0.015 ng, respectively. These results were obtained from a combination of high power (450 W), and extraction time (40 min). This indicates that using the ultrasound method with the correct parameters can increase the efficiency of the extraction results through the cavitation mechanism. Ultrasound method is an efficient and environmentally friendly extraction technique for molecular analysis process.

Copyright
© 2026 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits any noncommercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if you modified the licensed material. You do not have permission under this license to share adapted material derived from this chapter or parts of it.

Download article (PDF)

Volume Title
Proceedings of the 6th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BIOMIC 2025)
Series
Advances in Health Sciences Research
Publication Date
4 September 2026
ISBN
978-94-6239-762-0
ISSN
2468-5739
DOI
10.2991/978-94-6239-762-0_17How to use a DOI?
Copyright
© 2026 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits any noncommercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if you modified the licensed material. You do not have permission under this license to share adapted material derived from this chapter or parts of it.

Cite this article

TY  - CONF
AU  - Pramuja Baydillah
AU  - Ulfah Lailatul Khoiriah
AU  - Livya Safira Islam
AU  - Fadlilatul Taufany
AU  - Nurul Jadid
AU  - Andi Yudha Sasongko
AU  - Setiyo Gunawan
PY  - 2026
DA  - 2026/09/04
TI  - Pig DNA Extraction Using the Ultrasound Method for Halal Product Authentication
BT  - Proceedings of the 6th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BIOMIC 2025)
PB  - Atlantis Press
SP  - 258
EP  - 267
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6239-762-0_17
DO  - 10.2991/978-94-6239-762-0_17
ID  - Baydillah2026
ER  -