In Vitro Acetylcholinesterase Inhibition and Cytotoxic Effects of Selected Phenolic Compounds on Sh-Sy5y Cells

DOI

10.2991/978-94-6239-666-1_23How to use a DOI?

Keywords

Phenolic compounds; Acetylcholinesterase inhibition; SH-SY5Y cytotoxicity

Abstract

This study investigated the in vitro acetylcholinesterase (AChE) inhibitory activities and cytotoxic effects of twelve naturally derived phenolic compounds isolated from Scorzonera ketzkhowelii Sosn. ex Grossh. AChE inhibition was assessed by the Ellman colorimetric method, using galantamine as a reference inhibitor, while cytotoxicity was evaluated by the MTT assay at six serial dilutions ranging from 100 µM. Among the tested compounds, 3,5-di-O-caffeoylquinic acid and quercetin 3-O-α-arabinopyranoside exhibited notable AChE inhibitory activities, with IC₅₀ values of 0.56 µM and 0.89 µM, respectively, closely approaching the potency of galantamine (0.34 µM). Additionally, hydrangeic acid 4'-O-β-D-glucopyranoside, hydrangenol, and thunberginol F 7-O-β-D-glucopyranoside displayed moderate inhibitory activities, with IC₅₀ values ranging between 1.69 and 2.57 µM. In the cytotoxicity assays, hydrangeic acid 4'-O-β-D-glucopyranoside demonstrated the strongest effect on SH-SY5Y cells, yielding an IC₅₀ of 1.47 µM. This was followed by 3,5-di-O-caffeoylquinic acid, p-hydroxybenzaldehyde, and esculin, each showing IC₅₀ values below 3 µM, indicating considerable cytotoxic potential. Overall, these findings highlight the dual pharmacological promise of phenolic compounds from S. ketzkhowelii, which not only act as effective AChE inhibitors relevant to neurodegenerative disorders but also exhibit significant cytotoxic effects against neuroblastoma cells. This study provides a valuable basis for future investigations aimed at elucidating the molecular mechanisms underlying these activities and supports the continued exploration of phenolic structures from edible and medicinal plants as promising multitarget agents in both neurological and oncological therapeutic research.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Fatma Göç
AU  - Rabia Sare Yanikoğlu
AU  - Gülbahar Özge Alim Toraman
AU  - Aynur Sari
AU  - Serdar Makbul
AU  - Kamil Coşkunçelebi
PY  - 2026
DA  - 2026/05/07
TI  - In Vitro Acetylcholinesterase Inhibition and Cytotoxic Effects of Selected Phenolic Compounds on Sh-Sy5y Cells
BT  - Proceedings of the 5th International Conference on Research of Agricultural and Food Technologies (I-CRAFT 2025)
PB  - Atlantis Press
SP  - 269
EP  - 277
SN  - 3005-155X
UR  - https://doi.org/10.2991/978-94-6239-666-1_23
DO  - 10.2991/978-94-6239-666-1_23
ID  - Göç2026
ER  -