Detection of New Peptaibiotics from Mongolian Trichoderma Isolates

DOI

10.2991/978-94-6463-837-0_11How to use a DOI?

Keywords

Trichoderma citrinoviride peptaibiotics; UHPLC-ESI-HRMS; peptaibol; lipopeptaibol

Abstract

Trichoderma species are ubiquitous filamentous fungi with significant ecological versatility and impacts on agricultural and environmental systems. These fungi are predominantly found in soil, growing in the root system, as well as on wood and bark. Their diverse capabilities include enhancing plant growth, promoting nutrient uptake, and acting as biocontrol agents against a wide range of plant pathogens. They produce a plethora of bioactive secondary metabolites including peptaibiotics. These short, linear peptides are composed of unusual amino acid residues synthesized by non-ribosomal peptide synthetases. This study aimed to explore the peptaibiotics production of Trichoderma citrinoviride isolated from litter sources in Mongolia. An optimized HPLC separation coupled with high-resolution mass spectrometric (HRMS) detection was used to identify their peptaibiotic production. Based on the MS/MS fragmentation patterns, the key amino acid (α-aminoisobutyric acid, Aib) and amino alcohols were found in samples revealing the presence of specific peptaibiotics. As a result, 19- and 20-residue peptaibols, as well as 7-residue lipopeptaibols were identified from T. citrinoviride. Among these, certain peptaibols were identified as previously reported, while all the 19-residue peptaibols represent potentially novel compounds, proposed to be named brevilongibrachins. This study shows the metabolic potential of Mongolian Trichoderma species and contributes to the growing knowledge of fungal peptaibiotic diversity.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Nomuun Oyunbat
AU  - Enkh-Amgalan Jigjiddorj
AU  - Sarantuya Jargalsaikhan
AU  - Adiyadolgor Turbat
AU  - Dóra Balázs
AU  - Mónika Varga
AU  - Csaba Vágvölgyi
AU  - Tamás Papp
AU  - Tamás Marik
AU  - András Szekeres
PY  - 2025
DA  - 2025/09/17
TI  - Detection of New Peptaibiotics from Mongolian Trichoderma Isolates
BT  - Proceedings of the International Biological Conference in Mongolia 2025 (IBCM 2025)
PB  - Atlantis Press
SP  - 147
EP  - 167
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-837-0_11
DO  - 10.2991/978-94-6463-837-0_11
ID  - Oyunbat2025
ER  -