Establishment of an In Vitro Propagation Protocol for Spongiocarpella grubovii Ulzij., Subendemic Species of Mongolia

DOI

10.2991/978-94-6463-837-0_10How to use a DOI?

Keywords

Spongiocarpella grubovii Ulzij.; in vitro plantlets; propagation; ex vitro; subendemic

Abstract

Spongiocarpella grubovii Ulzij. is a species found in Mongolia that is threatened with extinction and is difficult to propagate using conventional methods. Therefore, in vitro propagation is expected to play an important role in the propagation of the species and in the rehabilitation of mining areas. In this study, a tissue culture protocol for Spongiocarpella grubovii was established. Significant contamination was found in the in vitro culture; however, the addition of a plant protection mixture (PPM) to the culture medium reduced the contamination to 60%. The medium in combination with α-naphthalene acetic acid (NAA), 6-benzyladenine (BA), zeatin and kinetin for shoot multiplication was analysed separately. The results showed that shoot multiplication on Murashige-Skoogs medium (MS) with 1.0 mg/L BA and 0.2 mg/L NAA was most effective and produced 4 shoots per explant after 4 weeks. For rooting of these propagated shoots, 0.1 and 1.0 mg/L indole-3-butyric acid (IBA), NAA and indole-3-acetic acid (IAA) were tested on full-strength MS, half-strength MS, full-strength Driver & Kuniyuki Walnut’s medium (DKW) and half-strength DKW medium. The results showed that 3.9 roots, 0.5 cm long, and 47% root induction were observed after 4 weeks in ½ MS medium containing 0.1 mg/L NAA. In vitro propagated shoots treated with 300 mg/L IBA solution successfully rooted 92% ex vitro and were developed for the first time for this species.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Tsolmon Munkhbayar
AU  - Munkhtstetseg Tserendagva
AU  - Bolortuya Ulziibat
AU  - Altantsetseg Balt
AU  - Oyunbileg Yungeree
PY  - 2025
DA  - 2025/09/17
TI  - Establishment of an In Vitro Propagation Protocol for Spongiocarpella grubovii Ulzij., Subendemic Species of Mongolia
BT  - Proceedings of the International Biological Conference in Mongolia 2025 (IBCM 2025)
PB  - Atlantis Press
SP  - 136
EP  - 143
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-837-0_10
DO  - 10.2991/978-94-6463-837-0_10
ID  - Munkhbayar2025
ER  -