Proceedings of the International Biological Conference in Mongolia 2025 (IBCM 2025)

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Targeted Disruption of the Orthohepadnavirus hominoidei S Gene Using CRISPR/Cas9 in HepG2 Cells

Authors
Erdene Baigali1, Sugar Lkhagvachuluun2, Tserendulam Batsukh2, Biligtsaikhan Tsolmon3, Enkhsaikhan Lkhagvasuren3, Budjav Jadamba3, Batjargal Batdorj1, Ochirkhuyag Baldorj1, Lkhagvasuren Damdindorj1, *
1National University of Mongolia, Ulaanbaatar, 14201, Mongolia
2Institute of Biology, Ulaanbaatar, 13330, Mongolia
3Mongolian National University of Medical Sciences, Ulaanbaatar, 14210, Mongolia
*Corresponding author. Email: lkhagvasuren.d@num.edu.mn
Corresponding Author
Lkhagvasuren Damdindorj
Available Online 17 September 2025.
DOI
10.2991/978-94-6463-837-0_17How to use a DOI?
Keywords
HBV dimer plasmid; HBV eradication; hepatitis B virus Orthohepadnavirus hominoidei; gene therapy
Abstract

Orthohepadnavirus hominoidei (formerly known as HBV) remains a significant global health concern, as chronic infection can lead to liver cirrhosis and hepatocellular carcinoma. One of the main challenges in treating this virus is that fragments of its DNA, once integrated into the host genome, remain intact and are not eliminated by current therapies. CRISPR/Cas9 technology has emerged as a promising genome-editing tool, with multiple studies demonstrating its ability to selectively target and cleave Orthohepadnavirus hominoidei DNA. In this study, we conducted targeted disruption experiments using the CRISPR/Cas9 system to target the S gene encoding the surface antigen of Orthohepadnavirus hominoidei. We successfully constructed the pX260-HBsAg vector and transfected it into HepG2 cells harboring a dimeric plasmid of the virus. Following the targeted disruption procedure, genomic DNA was isolated from the HepG2 cells, and the S gene was amplified by PCR. While the expected product of the S gene is 437 base pairs, the PCR yielded products of varying lengths, suggesting possible modifications at the target site.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Biological Conference in Mongolia 2025 (IBCM 2025)
Series
Advances in Biological Sciences Research
Publication Date
17 September 2025
ISBN
978-94-6463-837-0
ISSN
2468-5747
DOI
10.2991/978-94-6463-837-0_17How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Erdene Baigali
AU  - Sugar Lkhagvachuluun
AU  - Tserendulam Batsukh
AU  - Biligtsaikhan Tsolmon
AU  - Enkhsaikhan Lkhagvasuren
AU  - Budjav Jadamba
AU  - Batjargal Batdorj
AU  - Ochirkhuyag Baldorj
AU  - Lkhagvasuren Damdindorj
PY  - 2025
DA  - 2025/09/17
TI  - Targeted Disruption of the Orthohepadnavirus hominoidei S Gene Using CRISPR/Cas9 in HepG2 Cells
BT  - Proceedings of the International Biological Conference in Mongolia 2025 (IBCM 2025)
PB  - Atlantis Press
SP  - 238
EP  - 249
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-837-0_17
DO  - 10.2991/978-94-6463-837-0_17
ID  - Baigali2025
ER  -