Effect of Murashige and Skoog Medium Dose and Thiamin Concentration on in-vitro Microchannel Growth of Chrysanthemum Plantlet (Dendranthema grandiflora Tzvelev)

DOI

10.2991/978-94-6463-819-6_7How to use a DOI?

Keywords

Chrysanthemum; growth; Murashige and Skoog medium; thiamin; tissue culture

Abstract

Chrysanthemum (Dendranthema grandiflora Tzvelev) is a plant with a high economic and aesthetic value that is good for decorative ornamental plants. The high consumption level of Chrysanthemum encourages people to improve their productivity and quality with tissue culture techniques. The research aimed to determine the most appropriate Murashige and Skoog (MS) medium dose and the proper thiamin concentration and understand the interaction between the medium dose and thiamin concentration on the in-vitro growth of Chrysanthemum plantlets. The research method used was experimental with a Randomized Complete Design with two factors. The first factor was the dose of MS medium, which were 1, ¾, and ½ concentration. The second factor was the thiamin concentration of 1.5 mg/l, 2.5 mg/l, and 3.5 mg/l. Each treatment combination was repeated three times. The results showed that combining ½ MS medium and 2.5 mg/l thiamin could increase Chrysanthemum plantlets’ number of leaves and weight. The use of ½ MS medium produced the longest roots. The thiamin concentrations did not affect the microchannel growth of the Chrysanthemum plantlet.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - A. Wijayani
AU  - R. S. Lestari
AU  - N. W. Pratiwi
PY  - 2025
DA  - 2025/08/30
TI  - Effect of Murashige and Skoog Medium Dose and Thiamin Concentration on in-vitro Microchannel Growth of Chrysanthemum Plantlet (Dendranthema grandiflora Tzvelev)
BT  - Proceedings of the International Conference on Agriculture, Food, and Environment (ICAFE 2024)
PB  - Atlantis Press
SP  - 59
EP  - 64
SN  - 3005-155X
UR  - https://doi.org/10.2991/978-94-6463-819-6_7
DO  - 10.2991/978-94-6463-819-6_7
ID  - Wijayani2025
ER  -