Antiproliferative Activity of Methanol Fractions from the Stem Bark of Garuga floribunda Decne Against A549 Cells

DOI

10.2991/978-2-38476-410-5_26How to use a DOI?

Keywords

Antiproliferative Activity; mGaruga floribunda; A549 Cells

Abstract

The stem bark of Garuga floribunda Decne, commonly known as Buhu tree, has traditionally been used for treating tuberculosis, lung diseases, and cancer. It contains cyclic diarylheptanoids, primarily known for their diverse bioactivities such as anticancer, antioxidant, and anti-inflammatory properties. The research was undertaken due to the lack of prior studies on the anti-cancer potential of Buhu stem bark. This study aims to evaluate methanol extract from Buhu stem bark on A549 cells using the MTT assay. The study evaluated the toxicity of methanol extract obtained through maceration using the Brine Shrimp Lethality Test (BSLT) and its potential as an anticancer agent against A549 cancer cells using the MTT method. The methanol extract from Buhu stem bark confirmed the presence of tannins, saponins, flavonoids, alkaloids, and terpenoids. The results indicated that the methanol extract, tested using the BSLT method, exhibited toxicity with an LC50 value of 60.04 ppm. Furthermore, it was determined that it had antiproliferative activity. The methanol extract from Buhu stem bark showed diverse cell inhibition percentages at different extract concentrations. The methanol extract exhibited moderate cytotoxic activity with an IC50 value of 768.117 ppm. It is considered a potential anticancer agent.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Yuszda K. Salimi
AU  - Netty Ino Ischak
AU  - Arviani Arviani
AU  - Frisca Berliana Manjoe
AU  - Yosie Andriani
PY  - 2025
DA  - 2025/07/28
TI  - Antiproliferative Activity of Methanol Fractions from the Stem Bark of Garuga floribunda Decne Against A549 Cells
BT  - Proceedings of the 2nd International Conference on Sciences, Mathematics, and Education 2023 (ICOSMED 2023)
PB  - Atlantis Press
SP  - 260
EP  - 266
SN  - 2352-5398
UR  - https://doi.org/10.2991/978-2-38476-410-5_26
DO  - 10.2991/978-2-38476-410-5_26
ID  - Salimi2025
ER  -