Comparative Assessment of Fish Communities in Cengklik and Mulur Reservoirs Using eDNA Metabarcoding and Traditional Catch Methods

DOI

10.2991/978-94-6463-942-1_12How to use a DOI?

Keywords

Biodiversity; Fish; Metabarcoding; Monitoring; Reservoir

Abstract

Cengklik and Mulur reservoirs are two important inland water bodies in Surakarta regency, serving as water sources and habitats for diverse fish communities. Understanding the structure and composition of the fish communities is vital for biodiversity monitoring and resource management. This study aims to evaluate the performance of eDNA metabarcoding by comparing current eDNA fish community data with traditional catch inventory records in previous years. Water samples were collected using a 1 L sterilized bottle sampler at a depth of 0.5 m. The water samples were filtered with a 0.45 µm mixed cellulose ester filter paper, and the collected DNA was then extracted utilizing the DNA miniprep kit. Amplification of the 12S rRNA gene was carried out with the Mifish universal primer. Amplicon DNA was subsequently sequenced employing Illumina MiSeq 2x250. The research indicated that the DNA metabarcoding detected 13 fish species in Cengklik and 21 in Mulur. Both reservoirs exhibited a medium diversity index and a high dominance index. Moonlight gourami (Trichopodus microlepis) was identified as the dominant species in Cengklik, whereas Java barb (Barbonymus gonionotus) dominated the fish community in Mulur. Compared to traditional methods, eDNA metabarcoding identified more species in Cengklik (7 species) but slightly fewer in Mulur (23 species). Although differences in sampling periods may affect species presence due to temporal ecological shifts, this comparison demonstrates the potential and limitations of eDNA metabarcoding as an effective tool for fish biodiversity monitoring, provided that reference databases are comprehensive and sampling protocols are optimized.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

TY  - CONF
AU  - Wilsen Chuanata
AU  - Ari Susilowati
AU  - Agung Budiharjo
PY  - 2025
DA  - 2025/12/29
TI  - Comparative Assessment of Fish Communities in Cengklik and Mulur Reservoirs Using eDNA Metabarcoding and Traditional Catch Methods
BT  - Proceedings of the 3rd International Seminar on Fish and Fisheries Sciences (ISFFS 2025)
PB  - Atlantis Press
SP  - 133
EP  - 144
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-942-1_12
DO  - 10.2991/978-94-6463-942-1_12
ID  - Chuanata2025
ER  -