Differences in Hydroextraction Time on the Characteristics of Collagen Derived from Black Snapper Skin

DOI

10.2991/978-94-6463-942-1_21How to use a DOI?

Keywords

Black Snapper; Collagen Yield; Fish Skin; Hydroextraction Time

Abstract

The skin of black snapper (Lutjanus griseus) is a promising, environmentally sustainable, and economically viable alternative source of collagen. This study aimed to examine the effects of acetic acid concentration and hydroextraction duration on the quality characteristics of the extracted collagen. The extraction process consisted of three steps: First, the sample was treated with a 0.05 M NaOH solution (1:10, w/v) for 6 hours. Next, it was hydrolyzed using acetic acid (CH₃COOH) at concentrations of 0.05, 0.10, and 0.15 M (1:10, w/v) for 2 hours. Finally, it was hydroextracted in distilled water (1:2, w/v) at 40 ℃ for 1, 3, and 5 hours. A completely randomized design (CRD) featuring three replicates was employed for the experiment. The data were analyzed using ANOVA and Tukey’s post-hoc test at a 5% significance level. The results indicated that acetic acid concentration significantly affected the swelling degree (p < 0.05), with the highest swelling observed at 0.15 M, reaching 536.9%. Further optimization revealed that hydroextraction time also significantly impacted collagen yield and its chemical composition (p < 0.05). The optimal treatment was found to be a hydroextraction time of 5 hours. resulting in a yield of 5.39%, a protein content of 81.72%, a fat content of 0.74%, a moisture content of 5.32%, an ash content of 1.98%, and a pH of 6.68. In conclusion, these findings suggest that a concentration of 0.15 M acetic acid and a hydroextraction time of 5 hours are effective for extracting collagen from black snapper skin.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Yuliati H. Sipahutar
AU  - Baharudin Abdurazzak
AU  - Jaulim Sirait
AU  - Paulus P. R. Sitorus
AU  - Romauli J. Napitupulu
PY  - 2025
DA  - 2025/12/29
TI  - Differences in Hydroextraction Time on the Characteristics of Collagen Derived from Black Snapper Skin
BT  - Proceedings of the 3rd International Seminar on Fish and Fisheries Sciences (ISFFS 2025)
PB  - Atlantis Press
SP  - 278
EP  - 293
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-942-1_21
DO  - 10.2991/978-94-6463-942-1_21
ID  - Sipahutar2025
ER  -