Gastroprotective Effects of Propolis Against Ibuprofen-induced Gastric Injury: Ulcer Index and Histopathological Evaluation in Wistar Rat Model

DOI

10.2991/978-94-6239-836-8_3How to use a DOI?

Keywords

Propolis; Ibuprofen; Ulcer index; Gastroprotection; Histopathology; Wistar rats

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs), particularly ibuprofen, commonly induce gastric mucosal injury through cyclooxygenase inhibition, oxidative stress, and inflammation. Propolis, a natural product rich in polyphenols, possesses antioxidant and anti-inflammatory properties that may protect against gastric damage. This study investigated the gastroprotective effects of propolis in male Wistar rats with ibuprofen-induced gastric ulcers. Thirty rats were randomly divided into five groups (n = 6): normal control, ibuprofen-treated control (400 mg/kg/day), and three propolis pretreatment groups receiving 0.13, 0.26, or 0.52 mL/day before ibuprofen administration. Gastric injury was evaluated using the ulcer index and histopathological examination of gastric mucosal damage. Data were analyzed using the Kruskal–Wallis test, followed by Bonferroni-adjusted post hoc comparisons. Ibuprofen caused severe gastric injury, characterized by the highest ulcer index (16.50), epithelial erosion, and inflammatory cell infiltration. Propolis pretreatment reduced the ulcer index in a dose-dependent manner, with the lowest value observed in the 0.52 mL group (15.60). Histopathological analysis showed significantly lower gastric mucosal injury scores in the 0.26 and 0.52 mL groups than in the ibuprofen-treated control (p < 0.05). However, no significant differences were observed among propolis-treated groups. Propolis provided partial gastroprotection, although the tested doses did not achieve optimal efficacy.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Mulyati Sri Rahayu
AU  - Nora Maulina
AU  - Cut Sidrah Nadira
AU  - Sri Wahyuni
PY  - 2026
DA  - 2026/11/11
TI  - Gastroprotective Effects of Propolis Against Ibuprofen-induced Gastric Injury: Ulcer Index and Histopathological Evaluation in Wistar Rat Model
BT  - Proceedings of the3rd Malikussaleh International Conference on Health and Disaster Medicine 2026  (MICOHEDMED 2026)
PB  - Atlantis Press
SP  - 14
EP  - 25
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6239-836-8_3
DO  - 10.2991/978-94-6239-836-8_3
ID  - Rahayu2026
ER  -