Co-Fermentation of Glucose and Xylose by Gluconobacter oxydans for Simultaneous Production of Gluconic and Xylonic Acids

DOI

10.2991/978-94-6239-717-0_17How to use a DOI?

Keywords

Co-fermentation; Gluconic acid; Gluconobacter oxydans; Xylonic acid

Abstract

Gluconic and xylonic acids are increasingly recognized as valuable bio-based chemical building blocks due to their broad applications in food, pharmaceutical, and polymer industries. These organic acids are primarily derived from the oxidation of glucose and xylose, respectively those two major monosaccharides commonly present in lignocellulosic hydrolysates. In this study, the oxidative bacterium Gluconobacter oxydans (G.oxydans) was evaluated for its co-fermentation potential to simultaneously convert glucose and xylose into gluconic and xylonic acids. The results revealed that G.oxydans effectively fermented xylose even in the presence of glucose, highlighting its flexible metabolic response. Notably, co-fermentation led to the accumulation of up to 94.8 ± 6.7 g/L xylonic acid, with glucose consumption partially inhibited during this process. Interestingly, the production profile shifted based on glucose concentration: low glucose levels (25 g/L) favored xylonic acid accumulation, while higher glucose concentrations significantly enhanced gluconic acid production, reaching 113.3 ± 0.7 g/L within 120 hours at an initial glucose concentration of 75 g/L. The transition between acid products suggests a substrate-regulated metabolic switch within G.oxydans. This observation demonstrates the bacterium’s remarkable oxidative capacity and its suitability for valorizing sugar-rich feedstocks into multiple value-added products. The findings open avenues for developing efficient bioprocesses that simultaneously generate gluconic and xylonic acids, offering a sustainable route for integrated biorefinery applications.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ibnu Maulana Hidayatullah
AU  - Frederick Soetandar
AU  - Muhammad Sahlan
AU  - Masafumi Yohda
AU  - Naufal Baariq
AU  - Heri Hermansyah
PY  - 2026
DA  - 2026/06/25
TI  - Co-Fermentation of Glucose and Xylose by Gluconobacter oxydans for Simultaneous Production of Gluconic and Xylonic Acids
BT  - Proceedings of the 19th International Conference on Quality in Research (QiR 2025)
PB  - Atlantis Press
SP  - 231
EP  - 239
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6239-717-0_17
DO  - 10.2991/978-94-6239-717-0_17
ID  - Hidayatullah2026
ER  -