Tangential Flow Filtration-Based Isolation of UC-MSC Exosome: The Effect of Flowrate on Physical Properties

DOI

10.2991/978-94-6239-717-0_25How to use a DOI?

Keywords

Characterization; Exosome; Flowrate; UC-MSC; TFF

Abstract

Exosomes derived from mesenchymal stem cells (MSCs) have emerged as promising candidates in regenerative medicine due to their immunomodulatory properties. Tangential flow filtration (TFF) offers a scalable and GMP-complaint approach for exosome isolation, yet critical process parameters such as flowrate remain poorly understood. This study aimed to investigate the impact of TFF flowrate on the physical characteristics of UC-MSC Exosomes. UC-MSCs were cultured, condition media was collected and proceeded using TFF at two flowrates: low (10 mL/min) and high (30 mL/min) with a 500 kDa cutoff membrane. Physical characterization revealed that both groups produce exosomes within the expected size range (30-200 nm), but those isolated at low flowrate exhibited better monodispersity (PDI = 0.428 ± 0.01 vs. 0.520 ± 0.01) and greater colloidal stability, as indicated by more negative zeta potential (-27.9 ± 1.16 mV vs 18.4 ± 1.57 mV). These findings underscore the importance of TFF flowarate as critical parameters for maintaining exosome quality. Further study will be continued to quantify exosome particles, characterizing marker expression through flow cytometry (FACS), characterizing morphology through Cryo TEM and evaluating anti-inflammatory efficacy using in vitro assays.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Riski Imaniastuti
AU  - Rizky Ramadhani
AU  - Mohammad Wildan Abiyyi
AU  - Arry Yanuar
AU  - Fadlina Chany Saputri
AU  - Dede Heri Yuli Yanto
AU  - Jang Ho Lee
PY  - 2026
DA  - 2026/06/25
TI  - Tangential Flow Filtration-Based Isolation of UC-MSC Exosome: The Effect of Flowrate on Physical Properties
BT  - Proceedings of the 19th International Conference on Quality in Research (QiR 2025)
PB  - Atlantis Press
SP  - 348
EP  - 354
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6239-717-0_25
DO  - 10.2991/978-94-6239-717-0_25
ID  - Imaniastuti2026
ER  -