Metagenomic Analysis of Bacterial Diversity in an Acidic Sulfur Mud Pool in Kragilan, Banten Using Next-Generation Sequencing

DOI

10.2991/978-94-6239-717-0_22How to use a DOI?

Keywords

Acidophile; Bacterial Diversity; Metagenomics; Next-Generation Sequencing; Sulfur Mud Pool

Abstract

The exploration of microorganisms from extreme environments in Indonesia remains limited, despite the country’s richness in diverse and unique ecosystems. This study aimed to investigate the bacterial community diversity in a highly acidic sulfur mud pool in Kragilan, Banten, using the Oxford Nanopore Technologies Next-Generation Sequencing (NGS) platform. The mud sample was analyzed for its taxonomic composition and physiological characteristics based on literature. In situ measurements revealed a temperature of 28°C and a pH of 1.5. Metagenomic analysis identified a total of 157 bacterial genera and 412 species. The most dominant genus was Bacillus (31.01%), followed by Paenibacillus (19.42%) and Staphylococcus (16.53%). At the species level, Bacillus cereus (20.88%) showed the highest abundance. Despite the highly acidic environment, physiological analysis indicated that the majority of the identified bacteria were neutrophilic (53.70%) and mesophilic (75.67%), suggesting the presence of robust adaptation mechanisms to acidic conditions. Furthermore, several acidophilic species such as Acidibrevibacterium fodinaquatile, Alicyclobacillus disulfidooxidans, Acidicaldus organivorans, and Alicyclobacillus acidiphilus were also detected. These findings offer important preliminary insights into the complexity of bacterial communities inhabiting underexplored acidic environments in Indonesia and highlight the critical need for further research in such unique ecological niches. The exploration of acidic habitats holds significant potential for the discovery of novel acidophilic bacterial candidates with valuable industrial biotechnological applications, including biomining, the production of pH-stable enzymes, and environmental bioremediation.

Copyright

© 2026 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - David Herawan
AU  - Kenny Lischer
AU  - Fina Amreta Laksmi
AU  - Yudhi Nugraha
AU  - Elmega Gracia Elisabeth
PY  - 2026
DA  - 2026/06/25
TI  - Metagenomic Analysis of Bacterial Diversity in an Acidic Sulfur Mud Pool in Kragilan, Banten Using Next-Generation Sequencing
BT  - Proceedings of the 19th International Conference on Quality in Research (QiR 2025)
PB  - Atlantis Press
SP  - 302
EP  - 312
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6239-717-0_22
DO  - 10.2991/978-94-6239-717-0_22
ID  - Herawan2026
ER  -