Exploring the In-Vitro Antibacterial and Anti Tubercular Activity of Lamotrigine through Repurposing Approach

DOI

10.2991/978-94-6463-813-4_26How to use a DOI?

Keywords

Drug repurposing; Lamotrigine; Molecular Docking; Antibacterial activity; Antimycobacterial activity

Abstract

Tuberculosis ranks as the second deadliest infection caused by a single causative agent worldwide. Resistance towards the existing anti tubercular agents is the result of under diagnosis, phenotypic and genetic variation among patients and non-adherence to the current therapy. Drug discovery towards tuberculosis is unsuccessful in the past decade and gap is being filled by a computational approach, drug repurposing, which reintroduces the existing drugs with new indications. Currently, this approach is being actively employed to address a range of prevalent conditions such as tuberculosis, cancer, AIDS and COVID-19. The present study focuses on repurposing Lamotrigine, an anti epileptic drug, for its antibacterial and antimycobacterial activities. In silico molecular docking studies of Lamotrigine were conducted using Molegro Virtual Docker 6.0 with drug targets (PDBs) of Escherichia and Mycobacterium. The antibacterial activity was assessed using various bacterial strains including Bacillus subtilis(NCIM 2698), Pseudomonas aeruginosa (NCIM2639),Klebsiella pneumoniae (NCIM 2957), Escherichia coli (NCIM 2344) through the agar disc diffusion method with Gentamycin as the standard. Additionally, in vitro antimycobaterial activity was analysed against M. tuberculosis H37Rv, H37Ra, M. bovis utilising the microtitre plate alamar blue assay (MABA) with the standards Rifampin, Ciprofloxacin, Streptomycin, Pyrazinamide. The presence of both antibacterial and antimycobaterial activity of Lamotrigine was indicated by 20 and 14 mm diameter of zone of inhibition observed with Escherichia coli and Pseudomonas aeruginosa on agar plate as well as the development of blue colour in the microtitre well at a concentration of 1.6 μg mL⁻¹ during MABA.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Niharika Pedamallu
AU  - Manohar Babu Sitty
AU  - B. L. Raghunandhan
AU  - V. Ramya
AU  - B. Tripura
AU  - Bikash Ranjan Jena
PY  - 2025
DA  - 2025/08/13
TI  - Exploring the In-Vitro Antibacterial and Anti Tubercular Activity of Lamotrigine through Repurposing Approach
BT  - Proceedings of the International symposium on Sustainable Drug Design and Nanoparticle development: Quantum and Computational Perspectives (SDDNDQCP 2025)
PB  - Atlantis Press
SP  - 345
EP  - 356
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-813-4_26
DO  - 10.2991/978-94-6463-813-4_26
ID  - Pedamallu2025
ER  -