Proceedings of the 2025 International Conference on Chemical Engineering and Biological Science (CEBS 2025)

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Optimisation of GFP Recombinant Protein Expression in E. coli Using Response Surface Methodology

Authors
Xiaokai Zhang1, *
1School of Bioscience, University of Birmingham, Birmingham, UK
*Corresponding author. Email: zhangxk1221@outlook.com
Corresponding Author
Xiaokai Zhang
Available Online 28 August 2025.
DOI
10.2991/978-94-6463-829-5_27How to use a DOI?
Keywords
GFP; Recombinant protein expression; E. coli; Response Surface Methodology; protein solubility
Abstract

Green fluorescent protein (GFP), a commonly used reporter protein, is widely used in recombinant protein expression studies because of its unique fluorescent properties. In this study, the production of recombinant GFP protein in E. coli was used as an example to study the expression and solubility of GFP protein by varying temperature, IPTG concentration, induction time, galactose and lactose extracts. The ultimate goal is to optimize the expression of recombinant GFP protein in E. coli by analyzing the best expression conditions using the reaction surface methodology (RSM), which will provide a reference for industrial production. The results of RSM indicate that the optimal conditions for soluble GFP protein expression are: time: 4h, temperature: 35 ℃, yeast extract: 5g/L, IPTG concentration: 500uM, galactose: 5g/L.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2025 International Conference on Chemical Engineering and Biological Science (CEBS 2025)
Series
Advances in Engineering Research
Publication Date
28 August 2025
ISBN
978-94-6463-829-5
ISSN
2352-5401
DOI
10.2991/978-94-6463-829-5_27How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Xiaokai Zhang
PY  - 2025
DA  - 2025/08/28
TI  - Optimisation of GFP Recombinant Protein Expression in E. coli Using Response Surface Methodology
BT  - Proceedings of the 2025 International Conference on Chemical Engineering and Biological Science (CEBS 2025)
PB  - Atlantis Press
SP  - 276
EP  - 286
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-829-5_27
DO  - 10.2991/978-94-6463-829-5_27
ID  - Zhang2025
ER  -