De Novo Transcriptome Sequencing and Identification of Detoxification Enzyme Gene in Mulberry Pyralid (Glyphodes pyloalis) in Response to Nitenpyram Stress

DOI

10.2991/978-94-6463-829-5_10How to use a DOI?

Keywords

Glyphodes pyloalis; Toxicity; nitenpyram; transcriptome sequencing; detoxification enzyme gene

Abstract

The mulberry pyralid (Glyphodes pyloalis) is a major pest in mulberry fields, and its control has been a long-standing challenge. Investigating the toxic effects of nitenpyram on the mulberry pyralid and its detoxification mechanisms under nitenpyram stress is of great significance for pest management. In this study, the sublethal concentrations (LC30) of nitenpyram for the mulberry pyralid were determined at 24 and 48 hours to be 121.11 mg/L and 40.16 mg/L, respectively. Transcriptome sequencing was used to analyze Glyphodes pyloalis in the nitenpyram-treated and control groups, and high-quality sequencing data were obtained. The effective base ratio of each sample exceeded 95%, the Q30 ratio was about 95%, and the GC content was about 47%. After annotation, a large number of unigenes obtained functional annotation information in multiple databases. Significantly differentially expressed genes were screened, of which 289 were upregulated and 351 were downregulated (p<0.05 and |log₂FC|>1). Real-time quantitative PCR (RT-qPCR) validation demonstrated that the overexpression of detoxification enzyme genes including CYP4G15, CYP304, CYP337A2, GSTSigma2, UGT50A1, UGT40F1, ABCC3, and ABCF2 may be associated with the nitenpyram resistance in mulberry pyralid. This study not only provides novel insights into elucidating the molecular mechanisms of detoxification metabolism in G. pyloalis, but also establishes a theoretical foundation for developing targeted novel insecticides.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Yang Zhou
AU  - Wanglong Miao
AU  - Yiren Wang
AU  - Fuan Wu
PY  - 2025
DA  - 2025/08/28
TI  - De Novo Transcriptome Sequencing and Identification of Detoxification Enzyme Gene in Mulberry Pyralid (Glyphodes pyloalis) in Response to Nitenpyram Stress
BT  - Proceedings of the 2025 International Conference on Chemical Engineering and Biological Science (CEBS 2025)
PB  - Atlantis Press
SP  - 80
EP  - 86
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-829-5_10
DO  - 10.2991/978-94-6463-829-5_10
ID  - Zhou2025
ER  -