Proceedings of the 2025 2nd International Conference on Civil Engineering Structures and Concrete Materials (CESCM 2025)

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Experimental Study on Screening and Cultivation of Soil Reinforcement Strains Based on MICP Technology

Authors
En-zhe Deng1, 4, Qiang-qiang Miao1, 2, 3, 4, *, Qing-long Zhang1, *, Ji-zhong Gan2, 3, 4, Wan-zhi Cao2, 3, 4, Xiao-ming Zhu5, Kun Zhang2, Qing-qing Zhu2, Qing-xue Dang6, Tian-hui Chen6, Hong-juan Wu4, Zhang-hui Liu4
1Gansu Provincial Center of Technology Innovation for Health Monitoring and Security Assessment in Bridge and Tunnel, Lanzhou, 730124, China
2Key Laboratory of Green Engineering Materials and Low-Carbon Construction, Gansu, Lanzhou, 730000, China
3Industrial Research Institute of Prefabricated Buildings and Energy-Saving Materials, Gansu, Lanzhou, 747000, China
4College of Civil Engineering, Northwest Minzu University, Gansu, Lanzhou, 730000, China
5Gansu Province Communications Planning, Survey & Design Institute Co., Ltd., Gansu, Lanzhou, 747000, China
6Gannan Highway Development Center of Gansu Province, Gansu, Hezuo, 747000, China
*Corresponding author. Email: miaoqiang2008@qq.com
*Corresponding author. Email: qinglongzhang1101@163.com
Corresponding Authors
Qiang-qiang Miao, Qing-long Zhang
Available Online 22 December 2025.
DOI
10.2991/978-94-6463-932-2_22How to use a DOI?
Keywords
MICP; soil microorganisms; mineralization; urease
Abstract

Microbial Induced Calcium Carbonate Deposition (MICP) technology is a green and environmentally friendly method of soil consolidation by using microbial metabolite urease to promote the hydrolysis of urea to generate carbonate, which in turn combines with calcium ions to form calcium carbonate precipitation. However, the high cost of screening microorganisms used in the existing MICP technology limits its engineering application. The aim of this paper is to screen microorganisms with efficient urea hydrolysis from engineered site soils to reduce screening costs and improve adaptability. The microorganisms with urea hydrolysis ability were screened by soil dilution, nutrient agar medium culture, phenol red color reaction and pH determination, and their growth curves were determined by spectrophotometer. The results showed that the screened microorganisms exhibited significant growth advantages at 30℃ and 40% humidity, and their OD600 absorbance peaked at 12~13h and entered the stabilization period. Gram staining showed that the target strain was a Gram-positive bacterium, which was initially identified as Bacillus subtilis. The strains screened in this study have the advantages of low cost and adaptability compared to the traditional Bacillus subtilis. The results of this study provide the theoretical basis and technical support for the large-scale application of MICP technology in soil reinforcement engineering, and in the future, the classification of the strains can be further clarified and their mineralization efficacy can be optimized by gene sequencing.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 2025 2nd International Conference on Civil Engineering Structures and Concrete Materials (CESCM 2025)
Series
Advances in Engineering Research
Publication Date
22 December 2025
ISBN
978-94-6463-932-2
ISSN
2352-5401
DOI
10.2991/978-94-6463-932-2_22How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - En-zhe Deng
AU  - Qiang-qiang Miao
AU  - Qing-long Zhang
AU  - Ji-zhong Gan
AU  - Wan-zhi Cao
AU  - Xiao-ming Zhu
AU  - Kun Zhang
AU  - Qing-qing Zhu
AU  - Qing-xue Dang
AU  - Tian-hui Chen
AU  - Hong-juan Wu
AU  - Zhang-hui Liu
PY  - 2025
DA  - 2025/12/22
TI  - Experimental Study on Screening and Cultivation of Soil Reinforcement Strains Based on MICP Technology
BT  - Proceedings of the 2025 2nd International Conference on Civil Engineering Structures and Concrete Materials (CESCM 2025)
PB  - Atlantis Press
SP  - 207
EP  - 216
SN  - 2352-5401
UR  - https://doi.org/10.2991/978-94-6463-932-2_22
DO  - 10.2991/978-94-6463-932-2_22
ID  - Deng2025
ER  -