Pilot Study of Enzyme-Induced Calcium Carbonate Precipitation (EICP) Technique Based on Soybean and Potato Ureases
- DOI
- 10.2991/978-94-6463-932-2_23How to use a DOI?
- Keywords
- Enzyme-induced calcium carbonate precipitation (EICP); Urease activity; Plant urease; Urease extraction; Calcification test
- Abstract
Enzyme Induced Calcium Carbonate Precipitation (EICP) technology has many advantages over the traditional Microbial Induced Calcium Carbonate Precipitation (MICP) technology, such as the use of natural enzyme (urease) reduces the pollution of the environment, the economic benefits are better, and the construction is easy and controllable. This paper discusses the application of Enzyme Induced Calcium Carbonate Precipitation (EICP) technology in materials science and construction engineering. Urease was extracted from two high urease-producing plants, soybeans and potatoes, with a focus on comparing the activities of the two ureases under different conditions and their effects on calcium carbonate precipitation. The optimal conditions for urease extraction were determined through tests, and the effects of urea concentration, ethanol extract concentration, temperature, concentration ratio of urea to calcium chloride solution and enzyme-gel ratio on urease activity and calcium carbonate precipitation were analyzed. The results showed that the urease activity of soybean and potato increased and then decreased with the increase of urea concentration, and the optimal urea concentration was 1 mol/L and 1.2 mol/L, respectively; the urease activity of soybean was the highest and the extraction effect was the best when the concentration of ethanol extract was 20%, and the urease activity of potato was the highest and the extraction effect was the best when the concentration of ethanol extract was 30%; the urease activity of soybean was the highest and the extraction effect was the best when the concentration of ethanol extract was 30%; the urease activity of potato increased and the extraction effect was the best when the concentration of ethanol extract was 30%; and the urease activity of soybean increased with temperature and the extraction effect was the best when the concentration of ethanol extract was 30%. In the range of 5 ℃-50 ℃, soybean urease activity increased with increasing temperature, while potato urease activity decreased. In the calcification test, soybean urease performed better in inducing calcium carbonate precipitation, with the optimal urea to calcium chloride concentration ratio of 1:1.5, and the maximum amount of precipitation at an enzyme-gel ratio of 1:1. This study provides theoretical basis and data support for the application of EICP technology in the fields of reinforcement of building materials and soil improvement.
- Copyright
- © 2025 The Author(s)
- Open Access
- Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
Cite this article
TY - CONF AU - Zhang-hui Liu AU - Qiang-qiang Miao AU - Qing-long Zhang AU - Ji-zhong Gan AU - Wan-zhi Cao AU - Xiao-ming Zhu AU - Kun Zhang AU - Qing-qing Zhu AU - Qing-xue Dang AU - En-zhe Deng PY - 2025 DA - 2025/12/22 TI - Pilot Study of Enzyme-Induced Calcium Carbonate Precipitation (EICP) Technique Based on Soybean and Potato Ureases BT - Proceedings of the 2025 2nd International Conference on Civil Engineering Structures and Concrete Materials (CESCM 2025) PB - Atlantis Press SP - 217 EP - 231 SN - 2352-5401 UR - https://doi.org/10.2991/978-94-6463-932-2_23 DO - 10.2991/978-94-6463-932-2_23 ID - Liu2025 ER -