Proceedings of the International Conference on Agriculture, Food, and Environment (ICAFE 2024)

Growth Response and Yield of Brassica rapa sub sp. Pekinensis in the Treatment of Trichoderma harzianum Indigenous and Humic-Fulvic Acid with Pb Contaminated Planting Media

Authors
Oetami Dwi Hajoeningtijas1, *, Arif Prashadi Santosa1, Hamami Alfasani Dewanto1, Neni Damajanti2, Gina Aditiani3
1Agrotechnology Department, Agriculture and Fishery Faculty, Universitas Muhammadiyah Purwokerto, Jl. K. H. Ahmad Dahlan PO BOX 202, Banyumas, Central Jawa, Indonesia, 53182
2Chemical Enginering, Enginering and Sains Faculty, Universitas Muhammadiyah Purwokerto, Jl. K. H. Ahmad Dahlan PO BOX 202, Banyumas, Central Jawa, Indonesia, 53182
3Graduate of Agrotechnology Department, Agriculture and Fishery Faculty, Universitas Muhammadiyah Purwokerto, Jl. K. H. Ahmad Dahlan PO BOX 202, Banyumas, Central Jawa, Indonesia, 53182
*Corresponding author. Email: oetamidwihajoeningtyas@ump.ac.id
Corresponding Author
Oetami Dwi Hajoeningtijas
Available Online 30 August 2025.
DOI
10.2991/978-94-6463-819-6_17How to use a DOI?
Keywords
Brassica rapa sub sp. Pekinensis; Pb; humic-fulvic acid; Trichoderma harzianum indigenous
Abstract

According to the study findings, shallot planting fields in Brebes Regency were known to have Pb content above the tolerance limit, so they were used as a source of isolates and to test the effectiveness of the isolates obtained. Previous research has carried out isolation and identification of indigenous non-symbiotic fungi, including Trichoderma harzianum indigenous. Together with other non-symbiotic fungal isolates, they were then tested on a laboratory scale (in vitro) at various levels of heavy metal Pb contamination on PDA media. Isolate with the ability to survive in media with the highest concentration of heavy metals high, with the highest Tolerance Index value (8), namely Trichoderma harzianum. Efforts to increase effectiveness and further development were carried out by characterizing the physiology, biochemistry and further testing of selected Trichoderma harzianum. Apart from that, to increase the effectiveness of microbes as bioaccumulators, the administration of humic-fulvic acid was also tested. The problems studied in this research are: what is the influence of Trichoderma harzianum and/or humic-fulvic acid on the growth and yield of petsai (Brassica rapa subsp. Pekinensis) in Pb-contaminated planting media, and which of them have the best treatment effect on the growth and yield of petsai on planting media contaminated with Pb. The objectives of this research are as follows: to determine the effect of Trichoderma harzianum and/or humic-fulvic acid on the growth and yield of petsai on Pb-contaminated planting media, and knowing how to give them the best treatment for the growth and yield of petsai on planting media contaminated with Pb. Giving Tricoderma harzianum treatment, both F0 (without isolate) and F1 (with isolate) did not have a significant effect on the growth and yield of petsai. Giving humic-fulvic acid treatment consisting of F0 (0 ml humic-fulvic acid), F1 (750 ml humic-fulvic acid), F2 (1500 ml humic-fulvic acid) and F3 (2250 ml humic-fulvic acid) had a significant effect on the variables of leaf number and total root length. on petsai plants. The best combination of treatment with Trichoderma harzianum and humic-fulvic acid in the T1F1 combination is by administering isolate and a dose of humic-fulvic acid of 750 ml.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the International Conference on Agriculture, Food, and Environment (ICAFE 2024)
Series
Atlantis Highlights in Sustainable Development
Publication Date
30 August 2025
ISBN
978-94-6463-819-6
ISSN
3005-155X
DOI
10.2991/978-94-6463-819-6_17How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Oetami Dwi Hajoeningtijas
AU  - Arif Prashadi Santosa
AU  - Hamami Alfasani Dewanto
AU  - Neni Damajanti
AU  - Gina Aditiani
PY  - 2025
DA  - 2025/08/30
TI  - Growth Response and Yield of Brassica rapa sub sp. Pekinensis in the Treatment of Trichoderma harzianum Indigenous and Humic-Fulvic Acid with Pb Contaminated Planting Media
BT  - Proceedings of the International Conference on Agriculture, Food, and Environment (ICAFE 2024)
PB  - Atlantis Press
SP  - 189
EP  - 200
SN  - 3005-155X
UR  - https://doi.org/10.2991/978-94-6463-819-6_17
DO  - 10.2991/978-94-6463-819-6_17
ID  - Hajoeningtijas2025
ER  -