Identification of Bioactive Compounds and Testing The Activity of Takokak Fruit Ethanol Extract (Solanum Torvum L.) for Reducing Blood Cholesterol Levels for Hypercholesterolemia Patients

DOI

10.2991/978-94-6463-730-4_21How to use a DOI?

Keywords

takokak fruit; Solanum torvum L; hypercholesterolemia; ethanol extract

Abstract

Indonesia is a megabiodiversity country that has a lot of natural resources, especially plants, one of which is the takokak (Solanum torvum L.). Takokak contains bioactive compounds which are thought to have the potential to act as anti-hypercholesterolemia. Hypercholesterolemia is a condition where the concentration of cholesterol in the blood increases beyond normal and is a major risk factor for Coronary Heart Disease (CHD). This study aims to identify bioactive compounds from the ethanol extract of takokak fruit (Solanum torvum L.) and test its activity in reducing total cholesterol, LDL, HDL and triglyceride levels in mice (Mus musculus L). This research began with maceration and extraction of takokak fruit with ethanol solvent, then identified the structure and content of bioactive compounds in takokak fruit extract using phytochemical tests. Next, the takokak fruit extract activity test was carried out using a Completely Randomized Design (CRD) using 25 mice. The mice were divided into 5 groups and 5 repetitions, namely: negative control (without treatment), positive control (induced by cow’s brain), treatment group was induced by cow’s brain and then given takokak fruit extract 5.6 mg/25 gbb, 10.5 mg/25 gbb, and 17.5 mg/25 gbb. Observations were made on days 1, 15, and 29. Data were analyzed using ANOVA, with LSD (Least Significant Difference) further testing at a 5% significance level. The research results showed that from the phytochemical test, the ethanol extract of takokak fruit contained bioactive compounds of flavonoids, triterpenoids, tannins, saponins, and phenolics. Ethanol extract of fruit takokak (Solanum torvum L.) can reduce levels of total cholesterol, LDL, tiglycerides, and increase HDL in hypercholesterolemia mice (Mus musculus L.) with the most effective dose of 10.5 mg/25gbw.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Nuning Nurcahyani
AU  - Endang L. Widiastuti
AU  - Menti Manda Utama
PY  - 2025
DA  - 2025/05/27
TI  - Identification of Bioactive Compounds and Testing The Activity of Takokak Fruit Ethanol Extract (Solanum Torvum L.) for Reducing Blood Cholesterol Levels for Hypercholesterolemia Patients
BT  - Proceedings of the 5th International Conference on Applied Sciences, Mathematics, and Informatics (ICASMI 2024)
PB  - Atlantis Press
SP  - 245
EP  - 257
SN  - 2352-541X
UR  - https://doi.org/10.2991/978-94-6463-730-4_21
DO  - 10.2991/978-94-6463-730-4_21
ID  - Nurcahyani2025
ER  -