Quality and Proportion of Balinese Bull Sperm Using Percoll Density Gradient Centrifugation Sexing Method at Different Gradients with AndroMed® Diluent

DOI

10.2991/978-94-6463-670-3_21How to use a DOI?

Keywords

Andromed; Bali Cattle; Percoll Density Gradient Centrifugation; Sexing; Quality

Abstract

This study aimed to determine the quality and proportion of Bali Bull semen using the Percoll density gradient centrifugation (PDGC) method at different gradients with Andromed® diluent. The study was conducted at Singosari Artificial Insemination Center from December 2023 to February 2024. The experimental method used was a Randomized Group Design. The treatments in the study were T1: 10 Gradients (20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, and 65%), T2: 5 Gradients (20%, 30%, 40%, 50%, and 60%), and T3: 3 Gradients (20%, 40%, and 60%) with 10 replications. Statistical analysis showed highly significant differences (P < 0.01) in differences gradient treatments in the lower layer of individual motility. The results showed that the percentage of motility resulting from sexing in the lower layer obtained the highest value in T1 77.86%, while in the upper layer the highest percentage of motility was obtained in T3 which was 61.72% Meanwhile, in terms of viability, abnormalities, total motile spermatozoa and concentration, there were no differences (P > 0.05) in differences gradient treatments. While the proportion of spermatozoa in sexed X and Y, T1 = 77.6% and 84.8%; T2 = 75% and 81.8%; T3 = 75.8% and 79.1%. In conclusion, the quality of sexed semen was categorized as good, in terms of the parameters of motility, concentration and total motile spermatozoa.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Aditiya Wahyudi
AU  - Nanda Ayu Rahmawati
AU  - Aulia Puspita Anugra Yekti
AU  - Trinil Susilawati
AU  - Nurul Isnaini
AU  - Fardha Ad Durrun Nafis
PY  - 2025
DA  - 2025/04/18
TI  - Quality and Proportion of Balinese Bull Sperm Using Percoll Density Gradient Centrifugation Sexing Method at Different Gradients with AndroMed® Diluent
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 205
EP  - 212
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_21
DO  - 10.2991/978-94-6463-670-3_21
ID  - Wahyudi2025
ER  -