Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)

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Comparison of Extracellulase-producing Bacillus Isolated from the Digestive Tract of Muscovy Ducks (Cairina Moschata) and Native Chicken (Gallus Gallus Domesticus) as Probiotic Candidates for Poultry

Authors
Zaid Al Gifari1, Khairunnisah2, Fatimah Azh Zhahro Bagis5, Fitri Andriani5, Khairil Anwar5, Muhammad Aidil Fitriyan Fadjar Suryadi2, Alimuddin3, 5, Muhamad Amin4, Anwar Rosyidi5, I Wayan Wariata5, Made Sriasih5, Muhamad Ali5, *
1Laboratory of Animal Production, Faculty of Animal Sciences, University of Mataram, Mataram, 83125, Indonesia
2Graduate Program of Animal Resources, Faculty of Animal Sciences, University of Mataram, Mataram, 83125, Indonesia
3Faculty of Animal Sciences, University of Nahdlatul Wathan, Mataram, 82137, Indonesia
4Fish Nutrition Research Group, Department of Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Surabaya, 60115, Indonesia
5Laboratory of Biotechnology and Animal Product Processing, Faculty of Animal Sciences, University of Mataram, Mataram, 83125, Indonesia
*Corresponding author. Email: m_ali@unram.ac.id
Corresponding Author
Muhamad Ali
Available Online 18 April 2025.
DOI
10.2991/978-94-6463-670-3_36How to use a DOI?
Keywords
poultry; probiotics; Extracellular cellulase; Bacillus sp.; 16S rRNA gene sequencing
Abstract

Probiotics have widely been used as an alternative feed supplement in poultry, contributing to enhanced productivity and the maintenance of livestock health. This study aimed to compare several extracellular cellulase-producing Bacillus spp. Isolated from the digestive tract of muscovy ducks (Cairina moschata) and native chickens (Gallus gallus domesticus). A total of seven isolates were selected based on extracellular cellulase activity detected on carboxymethyl cellulose (CMC)-agar plates. Morphological, biochemical and molecular analyses were sequentially conducted to identify bacterial species. Three isolates exhibiting the best extracellular cellulase activity obtained from Muscovy Ducks (Cairina moschata) and native chickens (Gallus gallus domesticus) were selected and identified through 16S rRNA gene sequencing. The results showed that the A1 isolate was identified as Bacillus cereus strain SA275C, the A4 isolate as Bacillus cereus strain S8, and the A6 isolate as Bacillus subtilis strain IAM 12118. The three selected isolates showed different characteristics, including viability to digestive tract pH and antibacterial activity resulting in inhibition zones in isolates A1 and A4 of ± 0.5 mm, while isolate A6 was 1 mm against Staphylococcus aureus. In conclusion, the three selected isolates with the highest extracellular cellulase activity have potential as probiotic candidates for poultry.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
Series
Advances in Biological Sciences Research
Publication Date
18 April 2025
ISBN
978-94-6463-670-3
ISSN
2468-5747
DOI
10.2991/978-94-6463-670-3_36How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Zaid Al Gifari
AU  - Khairunnisah
AU  - Fatimah Azh Zhahro Bagis
AU  - Fitri Andriani
AU  - Khairil Anwar
AU  - Muhammad Aidil Fitriyan Fadjar Suryadi
AU  - Alimuddin
AU  - Muhamad Amin
AU  - Anwar Rosyidi
AU  - I Wayan Wariata
AU  - Made Sriasih
AU  - Muhamad Ali
PY  - 2025
DA  - 2025/04/18
TI  - Comparison of Extracellulase-producing Bacillus Isolated from the Digestive Tract of Muscovy Ducks (Cairina Moschata) and Native Chicken (Gallus Gallus Domesticus) as Probiotic Candidates for Poultry
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 351
EP  - 363
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_36
DO  - 10.2991/978-94-6463-670-3_36
ID  - AlGifari2025
ER  -