Nanoemulsion of Leea aequata (L.) Leaf Extract Enhances Antioxidant and Antibacterial Activities

DOI

10.2991/978-94-6463-962-9_32How to use a DOI?

Keywords

nanoemulsions; Leea aequata (L.); antioxidant; antimicrobial

Abstract

Leea aequata (L.), or Titanus leaf, is a tropical plant containing phenolic and flavonoid compounds with high antioxidant and antibacterial activity, but its low solubility and thermal instability limit its biological effectiveness. This research aims to develop and evaluate nanoemulsions of ethanol extract from Leea aequata (L.) leaves to improve their stability and biological activity against Pseudomonas aeruginosa. The nanoemulsions were formulated using a low-energy emulsification method with Tween 80, Span 80, sorbitol, and propylene glycol. The evaluation includes pH, viscosity, spreadability, particle size, as well as antioxidant (DPPH) and antibacterial (well diffusion) activity tests. The results showed that all formulas were stable without phase separation for 14 days at 25  ∘ C with a pH of 5.60 ± 0.30 – 6.55 ± 0.05 , viscosity of 1 , 613 ± 14.10 – 1 , 939 ± 2.08  cP, spreadability of 5.63 ± 0.45 – 6.56 ± 0.20  cm, and particle size of 62.3–65.8 nm. Antioxidant activity showed IC 50 values of 47.82 ppm (F1), 46.94 ppm (F2), and 43.19 ppm (F3), while antibacterial testing produced inhibition zones of 12.94 ± 0.15  mm (F1), 14.80 ± 0.25  mm (F2), and 16.60 ± 0.20  mm (F3), approaching the positive control gentamicin ( 18.40 ± 0.12  mm). These results indicate that nanoemulsions are capable of enhancing the stability, solubility, and biological effectiveness of Leea aequata extract, making them a potential natural ingredient for pharmaceutical and cosmetic topical preparations.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Vera Estefania Kaban
AU  - Roy Indrianto Bangar
AU  - Shintjia Dewi Agustan
AU  - Nurhanifah Sihotang
AU  - Irma Yohana Lumban Gaol
PY  - 2026
DA  - 2026/01/02
TI  - Nanoemulsion of Leea aequata (L.) Leaf Extract Enhances Antioxidant and Antibacterial Activities
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 322
EP  - 335
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_32
DO  - 10.2991/978-94-6463-962-9_32
ID  - Kaban2026
ER  -