Effectiveness of Keji Beling Leaf Extract on Malondialdehyde Levels in Serum and Heart of Cyanide-Induced Wistar Rats

DOI

10.2991/978-94-6463-760-1_36How to use a DOI?

Keywords

Cyanide; Keji Beling Leaves; Malondialdehyde levels; the serum; Heart

Abstract

Cyanide is a toxic compound that can cause disorders in the respiratory system, because the binding of cyanide to hemoglobin is higher than oxygen in the blood. If the cyanide level exceeds the endogenous antioxidant level, oxidative stress oxidative stress occurs which is characterized by increased levels of malondialdehyde (MDA). Keji beling leaves (Strobilanthes crispa) are antioxidants that can reduce MDA levels. The purpose of this study was to test phytochemicals, measure the antioxidant activity of methanol extract of keji beling leaves (MEKBL), and determine the effectiveness of MEKBL concentration in reducing MDA levels in serum and heart of cyanide-induced wistar rats. Method: This type of study used a post-test only with control group design. This study required 24 rats divided into 6 groups. All groups except Technical Control were exposed to cyanide 4 mg/kg BW of Rats. Negative control with cyanide, Positive control was given vitamin C, P1, P2, P3 were given MEKBL 5, 10, and 20 mg/kg BW of Rats for 10 days, then blood and heart organs were taken to analyze MDA levels. Antioxidant activity and MDA levels were measured using a Visible spectrophotometer, and data analysis used statistical tests. The results of the study obtained a yield of methanol extract of keji beling leaves of 10.3640%, with the results of phytochemical tests containing flavonoids, saponins, phenolics and tannins and the IC50 value 1.6150 ppm, classified as very strong antioxidant activity. MEKBL 20 mg/L can reduce serum MDA levels 72.53% and heart organs 71.02%. The results of the independent t-Test with p-value 0.079> 0.05, which means there is no difference between MDA levels in blood samples and heart. Conclusion: Methanol extract of keji beling leaves has the potential as an anti-oxidative stress agent in cyanide-induced rats.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Ana Hidayati Mukaromah
AU  - Stalis Norma Ethica
AU  - Fandhi Adi Wardoyo
AU  - Desti Fitriana
AU  - Chandra Eko Ardianto
AU  - Maria Fransiska Angeliana Nus Timba
PY  - 2025
DA  - 2025/07/03
TI  - Effectiveness of Keji Beling Leaf Extract on Malondialdehyde Levels in Serum and Heart of Cyanide-Induced Wistar Rats
BT  - Proceedings of the 3rd Lawang Sewu International Symposium on Medical and Health Sciences (LEWIS-MHS 2024)
PB  - Atlantis Press
SP  - 384
EP  - 400
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-760-1_36
DO  - 10.2991/978-94-6463-760-1_36
ID  - Mukaromah2025
ER  -