Cloning and Expression of Ruminococcus flavefaciens Cellulase-encoding Gene in E. coli as Feed Additive for Poultry

Khairil Anwar; Sukarne; Lalu Unsunnidhal; Muhammad Aidil Fitriyan Fadjar Suryadi; Zaid Gifari; Alimuddin; Muhamad Amin; Made Sriasih; Muhamad Ali

doi:10.2991/978-94-6463-670-3_33

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Cloning and Expression of Ruminococcus flavefaciens Cellulase-encoding Gene in E. coli as Feed Additive for Poultry

Authors

Khairil Anwar¹, Sukarne², Lalu Unsunnidhal³, Muhammad Aidil Fitriyan Fadjar Suryadi¹, Zaid Gifari⁴, Alimuddin¹^{, 5}, Muhamad Amin⁶, Made Sriasih¹, Muhamad Ali¹^{, *}

¹Laboratory of Biotechnology and Animal Product Processing, Faculty of Animal Sciences, University of Mataram, Mataram, 83115, Indonesia

²Laboratory of Animal Nutrition and Feed, Faculty of Animal Sciences, University of Mataram, Mataram, 83115, Indonesia

³Faculty of Food Science and Technology, University of Mataram, Mataram, 83115, Indonesia

⁴Laboratory of Animal Production, Faculty of Animal Sciences, University of Mataram, Mataram, 83115, Indonesia

⁵Faculty of Animal Sciences, University of Nahdlatul Wathan, Mataram, 82137, Indonesia

⁶Fish Nutrition Research Group, Department of Aquaculture, Faculty of Fisheries and Marine, Airlangga University, Surabaya, 60115, Indonesia

^*Corresponding author. Email: m_ali@unram.ac.id

Corresponding Author

Muhamad Ali

Available Online 18 April 2025.

DOI: 10.2991/978-94-6463-670-3_33 How to use a DOI?
Keywords: Carbohydrate-Metabolizing Enzyme; Poultry Industry; Ruminococcus flavefaciens Cellulase; Recombinant Protein; Codon Optimization; E. coli Expression System
Abstract: Carbohydrate-metabolizing enzymes are crucial in improving feed digestibility and efficiency in the poultry industry. Among these, cellulase, which breaks down cellulose, is particularly valued across various industries, especially in animal feed. However, producing cellulase in its native host, such as Ruminococcus flavefaciens, is often challenging due to complex cultivation requirements and limited yields. In this study, the cellulose-encoding gene from Ruminococcus flavefaciens was synthesized and expressed in Escherichia coli. The gene was codon-optimized and cloned into pET15b vector suitable for the E. coli expression system. The results showed a protein band at approximately 38 kDa, matching the expected size of the cellulase enzyme produced in E. coli. The optimized codon usage for E. coli significantly enhanced the soluble fraction of cellulase, which is crucial for large-scale production. In summary, the recombinant Ruminococcus flavefaciens cellulose-encoding gene was successfully expressed in E. coli. Although the expression of cellulase in E. coli was successful, further optimization is needed to increase yields and assess enzymatic activity.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
Series: Advances in Biological Sciences Research
Publication Date: 18 April 2025
ISBN: 978-94-6463-670-3
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-670-3_33 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Khairil Anwar
AU  - Sukarne
AU  - Lalu Unsunnidhal
AU  - Muhammad Aidil Fitriyan Fadjar Suryadi
AU  - Zaid Gifari
AU  - Alimuddin
AU  - Muhamad Amin
AU  - Made Sriasih
AU  - Muhamad Ali
PY  - 2025
DA  - 2025/04/18
TI  - Cloning and Expression of Ruminococcus flavefaciens Cellulase-encoding Gene in E. coli as Feed Additive for Poultry
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 323
EP  - 331
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_33
DO  - 10.2991/978-94-6463-670-3_33
ID  - Anwar2025
ER  -

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