Identification of IGF1 Gene Polymorphisms Using PCR-RFLP in Indigenous Goats of East Java

DOI

10.2991/978-94-6463-670-3_48How to use a DOI?

Keywords

IGF1 gene; Indigenous Goats; PCR-RFLP

Abstract

The objective of this study was to identify insulin growth factor (IGF-1) gene polymorphism using PCR RFLP technique in order to improve growth evaluation. The insulin-like growth factor 1 (IGF1) gene is a critical factor in the growth and development of goats. It influences a range of traits, including body size, milk production, and reproductive performance. This gene was therefore identified as a potential candidate gene. Blood samples were collected from 120 local goats of East Java, namely 43 Cross Etawa goats, 43 Senduro goats, and 43 Kacang goats. The DNA was extracted using the Geneaid DNA Purification Kit. The IGF-1 polymorphisms were determined by the PCR-RFLP method, employing the HaeIII restriction enzyme. The primers for the 398 bp long IGF1 gene are forward 5’ GGAATCGTGGATGAGTGC 3’ and reverse 5’ CACAGCAGTAGAAGGGAG 3’. The results demonstrated that the local goat population in East Java is in Hardy-Weinberg equilibrium. The conclusion of this study is that the IGF-1 gene in local goats in East Java is polymorphic, and this diversity information can be employed for association studies with growth traits, litter size, and twinning rates.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Wike Andre Septian
AU  - Ari Ardiantoro
AU  - Kuswati
AU  - Muhaimin Rifa’i
AU  - Mohammad Alessandro Nalaprily Bagus Prasetya
AU  - Suyadi
PY  - 2025
DA  - 2025/04/18
TI  - Identification of IGF1 Gene Polymorphisms Using PCR-RFLP in Indigenous Goats of East Java
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 452
EP  - 460
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_48
DO  - 10.2991/978-94-6463-670-3_48
ID  - Septian2025
ER  -