Quality and Proportion of X and Y Spermatozoa Using Different Sexed Methods in Friesian Holstein Cattle

DOI

10.2991/978-94-6463-670-3_8How to use a DOI?

Keywords

Albumin Sedimentation; Friesian Holstein; Percoll Density Gradient Centrifugation; Sexed Semen; Semen Quality; Spermatozoa Proportion

Abstract

Sexing process can damage the spermatozoa membrane, which causes a decrease in the quality of the spermatozoa, so an appropriate method is needed to protect the spermatozoa membrane and maintain its quality. This study aimed to determine the quality of frozen sexed semen using Percoll Density Gradient Centrifugation (PDGC) method from Singosari Artificial Insemination Center and frozen sexed semen using the Albumin Sedimentation (AS) method from Lembang Artificial Insemination Center. Each type of sample was repeated ten times. The variables used were individual motility, viability, abnormality, concentration, total motile spermatozoa (TMS) and proportion of X and Y spermatozoa. Data analysis used in this research was a Randomized Group Design, Duncan Multiple Range Test and Chi Square Test. The results of unsexed frozen semen, sexed frozen semen using PDGC method, and sexed frozen semen using AS method showed that individual motility values were (46.0, 41.1, 40.6%), viability (66.1, 59.8, 61.4%), concentration (34.7, 28.5, 12.6 million/straw) and TMS (16.0, 11.7, 5.1 million/straw). The results showed that frozen sexed semen using the PDGC method has qualified the Indonesian National Standard so it can be distributed and feasible to be inseminated. Frozen sexed semen using the AS method on spermatozoa concentration and TMS value has not qualified for the Indonesian National Standard.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Firlia Safa
AU  - Amir Firdaus
AU  - Putri Utami
AU  - Aulia Puspita Anugra Yekti
AU  - Achadiah Rachmawati
AU  - Trinil Susilawati
PY  - 2025
DA  - 2025/04/18
TI  - Quality and Proportion of X and Y Spermatozoa Using Different Sexed Methods in Friesian Holstein Cattle
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 76
EP  - 85
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_8
DO  - 10.2991/978-94-6463-670-3_8
ID  - Safa2025
ER  -