Polymorphism Analysis of BMP15 and GDF9 Genes in Dorper Sheep Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Technique

DOI

10.2991/978-94-6463-670-3_30How to use a DOI?

Keywords

Dorper Sheep; BMP15 Gene; GDF9 Gene; Litter Size; PCR-RFLP

Abstract

This study examined BMP15 and GDF9 gene polymorphisms in Dorper sheep and their potential link to prolificacy traits. Blood samples from 17 Dorper ewes with recorded litter sizes were analyzed using PCR-RFLP with HAEIII and HhaI restriction enzymes for BMP15 and GDF9 genes, respectively. Despite observed litter size variations (70.59% single births, 29.41% twin births), PCR-RFLP analysis revealed monomorphic patterns for both genes across all samples. All individuals exhibited wild-type genotypes, with no genetic variation detected at the examined restriction sites. Genotype and allele frequencies were 1 for both genes, resulting in zero heterozygosity and polymorphic information content (PIC) values. These findings suggest that the PCR-RFLP method with the selected restriction enzymes may not effectively detect prolificacy-related genetic variations in Dorper sheep. The discrepancy between genetic homogeneity and phenotypic variability in litter size indicates that other genes or molecular mechanisms may influence prolificacy traits in this breed. Further research using larger sample sizes, alternative genetic markers, and more sensitive techniques is recommended to elucidate the genetic basis of prolificacy in Dorper sheep.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Asrullah As
AU  - Tri Eko Susilorini
AU  - Wike Andre Septian
AU  - Chairdin Dwi Nugraha
AU  - Rafika Febriani Putri
AU  - Ari Ardiantoro
AU  - Suyadi
PY  - 2025
DA  - 2025/04/18
TI  - Polymorphism Analysis of BMP15 and GDF9 Genes in Dorper Sheep Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Technique
BT  - Proceedings of the 5th International Conference on Environmentally Sustainable Animal Industry (ICESAI 2024)
PB  - Atlantis Press
SP  - 292
EP  - 303
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-670-3_30
DO  - 10.2991/978-94-6463-670-3_30
ID  - As2025
ER  -