The Use of Ethylene Glycol Cryoprotectant with Varying Concentrations on the Integrity of the Plasma Membrane and DNA Damage in Gaga Chickens Semen

Muhammad Robbaani; Khaeruddin Khaeruddin; Achadiah Rachmawati; Ita Wahyu Nursita; Sri Wahjuningsih

doi:10.2991/978-94-6463-880-6_11

<Previous Article In Volume

Next Article In Volume>

The Use of Ethylene Glycol Cryoprotectant with Varying Concentrations on the Integrity of the Plasma Membrane and DNA Damage in Gaga Chickens Semen

Authors

Muhammad Robbaani¹^{, *}, Khaeruddin Khaeruddin², Achadiah Rachmawati¹, Ita Wahyu Nursita¹, Sri Wahjuningsih¹

¹Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia

²Faculty of Agriculture, Universitas Muhammadiyah Sinjai, Sinjai, 92615, Indonesia

^*Corresponding author. Email: banihk2@gmail.com

Corresponding Author

Muhammad Robbaani

Available Online 31 October 2025.

DOI: 10.2991/978-94-6463-880-6_11 How to use a DOI?
Keywords: gaga chickens; ethylene glycol; plasma membrane integrity; DNA damage; sperm
Abstract: This study aimed to assess the effectiveness of cryoprotectant ethylene glycol with graded concentrations on cryopreserved Gaga chicken semen. The treatment repetitions in this study were ten times, included Dimethyl Sulfoxide (DMSO) 7% as control, Ethylene Glycol 5%, Ethylene glycol 7%, and Ethylene glycol 9%. The research procedures included semen collection, semen dilution, semen packaging, prefreezing and freezing. The variables analyzed were plasma membrane integrity and DNA damage before freezing and after thawing, as well as a decrease in plasma membrane integrity and an increase in DNA damage after thawing. Data were analyzed using analysis of variance (ANOVA). The results showed that there was no significant effect of all treatments on the variables evaluated. This study found that there was no significant effect on all variables with an average plasma membrane integrity after thawing getting the results of 48-55% and the average DNA damage was 6-8%.
Copyright: © 2025 The Author(s)
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

<Previous Article In Volume

Next Article In Volume>

Volume Title: Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
Series: Advances in Biological Sciences Research
Publication Date: 31 October 2025
ISBN: 978-94-6463-880-6
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-880-6_11 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Muhammad Robbaani
AU  - Khaeruddin Khaeruddin
AU  - Achadiah Rachmawati
AU  - Ita Wahyu Nursita
AU  - Sri Wahjuningsih
PY  - 2025
DA  - 2025/10/31
TI  - The Use of Ethylene Glycol Cryoprotectant with Varying Concentrations on the Integrity of the Plasma Membrane and DNA Damage in Gaga Chickens Semen
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 95
EP  - 103
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_11
DO  - 10.2991/978-94-6463-880-6_11
ID  - Robbaani2025
ER  -

download .riscopy to clipboard