The Effectiveness of Sunkist Orange (Citrus sinensis) Peel Extract Cream on Collagen Density in the Wound Healing Process of Excisional Wounds in White Rats (Rattus norvegicus)

DOI

10.2991/978-94-6463-962-9_22How to use a DOI?

Keywords

Sunkist orange peel; Citrus sinensis; collagen density; Excision wound; Cream

Abstract

Sunkist orange peel (Citrus sinensis (L.) Osbeck) is known to contain various bioactive compounds such as flavonoids, vitamin C, vitamin A, tannins, saponins, and antioxidants, which have potential to support wound healing through anti-inflammatory, antioxidant, and tissue regeneration mechanisms. This study aimed to determine the effect of Sunkist orange peel extract cream on collagen density in the skin tissue of rats with excision wounds. This experimental study used a post-only control group design, involving 25 rats divided into five groups: a negative control group (cream base), a positive control group (standard cream/Ponds), and three treatment groups receiving 10%, 15%, and 20% Sunkist orange peel extract cream. The extract was prepared by maceration method. The treatment was given daily for four weeks. After treatment, the rats’ skin tissue was examined histologically to assess collagen density using a semi-quantitative scoring system. The results showed no significant difference in collagen density scores among the treatment and control groups ( p = 0.075 ). However, there was a tendency for an increased median collagen density score in the 20% treatment group (median 4) compared to the control group (median 3). In conclusion, administration of Sunkist orange peel extract cream did not show a significant difference in increasing collagen density but indicated potential wound healing activity. Further studies with dose variations and additional parameters are recommended to optimize the potential of Sunkist orange peel extract as a wound healing agent.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Angelika Sio Siagian
AU  - Maya Sari Mutia
AU  - Yolanda Eliza Putri Lubis
PY  - 2026
DA  - 2026/01/02
TI  - The Effectiveness of Sunkist Orange (Citrus sinensis) Peel Extract Cream on Collagen Density in the Wound Healing Process of Excisional Wounds in White Rats (Rattus norvegicus)
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 222
EP  - 228
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_22
DO  - 10.2991/978-94-6463-962-9_22
ID  - Siagian2026
ER  -