Phytochemical Identification and Antibacterial Activity of Castanopsis costata Leaves Extract Against Staphylococcus aureus

DOI

10.2991/978-94-6463-962-9_21How to use a DOI?

Keywords

Castanopsis costata; phytochemical; antibacterial; Staphylococcus aureus

Abstract

Castanopsis costata, a species from the Fagaceae family, has been traditionally used for medicinal purposes, yet its antibacterial potential remains largely unexplored. This study aimed to evaluate the phytochemical constituents and antibacterial activity of C. costata leaf extract against Staphylococcus aureus. Leaves were extracted using ethanol and diluted to various concentrations (0.078125%–50%) in DMSO. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, terpenoids, and phenolics, while saponins were absent. Antibacterial activity was assessed using the Kirby–Bauer disc diffusion method on Mueller Hinton Agar inoculated with S. aureus (1.5  ×  10 8  CFU/mL). Inhibition zones were measured after incubation at 37 ∘ C for 24 hours. The extract exhibited concentration-dependent antibacterial activity, with the largest zone at 50% concentration (14.2 ± 0.12 mm). No inhibition was observed at concentrations ≤ 0.3125%. Chloramphenicol (positive control) produced a zone of 23.56 ± 2.43 mm, while DMSO (negative control) showed no activity. These findings suggest that C. costata leaf extract possesses moderate antibacterial activity and contains several bioactive compounds, supporting its potential as a natural antimicrobial agent. Further studies are needed to isolate active constituents and explore their mechanisms of action.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Arna Fransisca Millyanti Purba
AU  - Gusbakti Rusip
AU  - Maya Sari Mutia
PY  - 2026
DA  - 2026/01/02
TI  - Phytochemical Identification and Antibacterial Activity of Castanopsis costata Leaves Extract Against Staphylococcus aureus
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 212
EP  - 221
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_21
DO  - 10.2991/978-94-6463-962-9_21
ID  - Purba2026
ER  -