Supplementation Evaluation of Centella asiatica Extract Combined with Ascorbic Acid on Hemoglobin, Glycemic Regulation, and Neutrophil–Lymphocyte Ratio in Staphylococcus aureus–Induced Sepsis Rats

DOI

10.2991/978-94-6463-962-9_23How to use a DOI?

Keywords

Centella asiatica; Ascorbic acid; Sepsis; hemoglobin; Neutrophil–lymphocyte ratio (NLR)

Abstract

Sepsis induced by Staphylococcus aureus triggers widespread inflammation, oxidative stress, and metabolic disturbance, leading to hematologic impairment, altered glycemic control, and immune dysregulation. This study evaluates the effects of Centella asiatica extract combined with ascorbic acid on hemoglobin, glycemic regulation, and neutrophil–lymphocyte ratio (NLR) in septic rats. Thirty Wistar rats were randomly assigned into six groups: normal control, septic negative control, septic positive control (doxycycline), C. asiatica alone, ascorbic acid alone, and combination therapy. Sepsis was induced via intraperitoneal injection of S. aureus, and treatments were administered orally over six consecutive days. Compared to the septic control, both single and combination treatments significantly improved hemoglobin levels and reduced NLR (p ≤ 0.05), indicating enhanced hematologic and immunologic responses. The combination treatment also moderated fasting blood glucose, though changes in glycated hemoglobin (HbA1c) were not statistically significant consistent with the short duration of intervention. Findings suggest that C. asiatica plus ascorbic acid supplementation may serve as a promising adjuvant therapy in bacterial sepsis by mitigating inflammation, supporting hematologic function, and aiding metabolic regulation. Further long-term studies are warranted to validate these effects.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Sabar Hamonangan Victorianus Napitu
AU  - Gusbakti Rusip
AU  - Maya Sari Mutia
PY  - 2026
DA  - 2026/01/02
TI  - Supplementation Evaluation of Centella asiatica Extract Combined with Ascorbic Acid on Hemoglobin, Glycemic Regulation, and Neutrophil–Lymphocyte Ratio in Staphylococcus aureus–Induced Sepsis Rats
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 229
EP  - 238
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_23
DO  - 10.2991/978-94-6463-962-9_23
ID  - Napitu2026
ER  -