Study of Bioactive Compounds and Antibacterial Properties of Mitragyna speciosa Leaf Extract Against Staphylococcus aureus

DOI

10.2991/978-94-6463-962-9_24How to use a DOI?

Keywords

Mitragyna speciosa; phytochemical screening; antibacterial; Staphylococcus aureus; disc diffusion assay.

Abstract

The increasing resistance of pathogenic bacteria such as Sta-phylococcus aureus to conventional antibiotics highlights the need for alternative antimicrobial agents derived from natural sources. This study aimed to analyze the phytochemical constituents and evaluate the antibacterial activity of Mitragyna speciosa leaf extract against S. aureus. Phytochemical screening confirmed the presence of several bioactive compounds, including alkaloids, flavonoids, tannins, terpenoids, saponins, and phenolics, all of which are known to contribute to antimicrobial and therapeutic properties. The antibacterial activity was assessed using the disc diffusion method across a range of extract concentrations. Results demonstrated a concentration-dependent inhibition of S. aureus, with the highest zone of inhibition observed at 50% concentration (13.2 ± 1.83 mm). Moderate activity was noted at 40% (12.4 ± 1.43 mm) and 30% (11.3 ± 1.34 mm), while concentrations at or below 0.625% showed no observable effect. The standard antibiotic chloramphenicol (positive control) produced a zone of 23.56 ± 2.43 mm, while the negative control (DMSO) showed no inhibition. These findings suggest that M. speciosa leaves contain active phytochemicals with promising antibacterial properties, which may serve as a basis for the development of novel natural antimicrobial formulations.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Edlin Edlin
AU  - Gusbakti Rusip
AU  - Maya Sari Mutia
PY  - 2026
DA  - 2026/01/02
TI  - Study of Bioactive Compounds and Antibacterial Properties of Mitragyna speciosa Leaf Extract Against Staphylococcus aureus
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 239
EP  - 246
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_24
DO  - 10.2991/978-94-6463-962-9_24
ID  - Edlin2026
ER  -