Effectiveness of Methanol Extract of Andaliman (Zanthoxylum acanthopodium DC.) on the Liver of Male Wistar Rats Induced with Benzo[a]pyrene

DOI

10.2991/978-94-6463-962-9_27How to use a DOI?

Keywords

Benzo[a]pyrene; Zanthoxylum acanthopodium; Liver enzymes; Histopathology; Hepatoprotection

Abstract

Benzoapyrene is a polycyclic aromatic hydrocarbon that can cause liver injury through oxidative stress and has carcinogenic potential. Zanthoxylum acanthopodium, known as andaliman, is a traditional spice from North Sumatra containing bioactive compounds with antioxidant and hepatoprotective activity. This study aimed to evaluate the hepatoprotective effect of the methanolic extract of Zanthoxylum acanthopodium in male Wistar rats exposed to Benzo[a]pyrene. A total of 25 male rats were divided into 5 groups. On day 1, all groups received a single intraperitoneal injection of Benzo[a]pyrene at 25 mg/kg body weight. Group 1 served as a control and received no further treatment, group 2 was given sesame oil, while groups 3 to 5 were administered the extract orally at doses of 200, 300, and 400 mg/kg body weight for 28 consecutive days. On day 30, serum levels of liver enzymes were measured, and liver tissues were examined histologically. Benzo[a]pyrene significantly increased serum transaminase levels and caused hepatocellular degeneration, while administration of the extract reduced these changes in a dose-dependent manner. The dose of 300 mg/kg body weight showed the most effective protection, with near-normal enzyme levels and minimal tissue changes, whereas higher doses showed less benefit. In conclusion, the methanolic extract of Zanthoxylum acanthopodium at 300 mg/kg body weight was the most effective hepatoprotective dose against Benzo[a]pyrene-induced liver injury.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Linda Chiuman
AU  - Thania Raj Kaur
AU  - Daisy Natalie Lowis
AU  - Vindelin Sanoesi
AU  - Brandon Lim
AU  - Davin Hendrik Salim
AU  - Suandy Suandy
PY  - 2026
DA  - 2026/01/02
TI  - Effectiveness of Methanol Extract of Andaliman (Zanthoxylum acanthopodium DC.) on the Liver of Male Wistar Rats Induced with Benzo[a]pyrene
BT  - Proceedings of the 2nd International Conference on Lifestyle Diseases and Natural Medicine (ICOLIFEMED 2025)
PB  - Atlantis Press
SP  - 267
EP  - 281
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-962-9_27
DO  - 10.2991/978-94-6463-962-9_27
ID  - Chiuman2026
ER  -