Revealing Potency of Bioactive Compounds as Anti Leptospira from Phyllanthus niruri

DOI

10.2991/978-94-6463-760-1_20How to use a DOI?

Keywords

anti Leptospira sp; Phyllanthus niruri; Phyllanthin; LipL-32

Abstract

In the last 3 years, cases of Leptospirosis have increased in Indonesia. There were reports of resistance to sulfamethoxazole and fosfomycin (types of antibiotics) against a small number of Leptospira serovars. Phyllanthus amarus is morphologically and phytochemically similar to P. niruri. P. amarus is known to have anti-Leptospira sp activity. The aims of this research was to prove that P. niruri extract has anti-Leptospira sp activity. Sample herbs were collected from the Tawangmangu Research and Development Center for Medicinal Plants and Traditional Medicine. The anti Lepto invirto test was carried out at the Banjarnegara Public Health Laboratory Center. Sample for molecular docking was collected from Pubchem (NCBI, USA) and Protein Data Bank (PDB), then drug-likeness analysis using Lipinski rule of five in SCFBIO web server and bioactive probability analysis of bioactive compounds. Furthermore, the blind docking method was performed using PyRx 0.8 software to determine the binding activity and molecular interaction by Biovia discovery studio visualizer. The anti-Leptospira sp in vitro test shows that methanol extract from Phyllanthus niruri has potential as an anti-Leptospira sp. In silico testing using molecular docking showed that the characteristic compound for P. niruri, namely phyllanthin, was predicted to be a better anti-Leptospira sp candidate than hypophyllanthin because it has a more negative binding energy. Phylanthin can be used as a candidate for anti-Lepospira sp with its inhibitory activity.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Dewi Marbawati
AU  - Tri Rini Nuringtyas
AU  - Niko Prasetyo
AU  - Tri Joko Raharjo
PY  - 2025
DA  - 2025/07/03
TI  - Revealing Potency of Bioactive Compounds as Anti Leptospira from Phyllanthus niruri
BT  - Proceedings of the 3rd Lawang Sewu International Symposium on Medical and Health Sciences (LEWIS-MHS 2024)
PB  - Atlantis Press
SP  - 220
EP  - 232
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-760-1_20
DO  - 10.2991/978-94-6463-760-1_20
ID  - Marbawati2025
ER  -