Bioaugmentation of Soil Artificially Polluted with Acetaminophen and Hydroxychloroquine

DOI

10.2991/978-94-6463-876-9_11How to use a DOI?

Keywords

Acetaminophen; Bioaugmentation; Consortia; Hydroxychloroquine; Soil

Abstract

Bioaugmentation is a promising technique for repairing sites contaminated with refractory chemicals like drugs such as acetaminophen and hydroxychloroquine. Microorganisms have been shown to be effective in breaking down drugs in contaminated areas. Here we report the bioremediation of soil contaminated with acetaminophen (1000 ppm) and hydroxychloroquine (100 ppm) in separate batches. A total of 4 sets (2 test and 2 control) were prepared for each drug using sterile and nonsterile soils. Based on the previous in vitro biodegradation studies, selection of the strains was made. Kosakonia cowanii JCM 10956(T) (GenBank accession no. OQ733302.1), Bacillus velezensis (GenBank accession no. OQ932827.1), Pseudomonas aeruginosa (GenBank accession no. OQ992198.1), Bacillus subtilis subsp. Subtilis (GenBank accession no. OQ733302.1) and Paenibacillus validus (GenBank accession no. OQ749901.1) strains were used in consortia for the bioaugmentation of soil spiked with acetaminophen. For soil spiked with Hydroxychloroquine, consortia of Kosakonia cowanii JCM 10956(T), Bacillus velezensis, Pseudomonas aeruginosa, and Paenibacillus validus strains were used. For acetaminophen-polluted soil, 89% and 95% degradation were reported in sterile and nonsterile bioaugmented soil, respectively. Whereas, for hydroxychloroquine-polluted soil, 90% and 98% degradation were estimated. Natural degradation of the hydroxychloroquine was detected in the control soil. Based on the HPLC analysis, it can be concluded that nonsterile drug-polluted soil amended with microbial consortia showed an effective decrease in the drugs after 10 days of incubation compared to the sterile soil with and without microorganisms. This study gives new insights into the field of the insite soil remediation to eliminate pharma pollutants.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Meghmala S. Waghmode
AU  - Pragati S. Abhyankar
AU  - Dipak K. Sahoo
AU  - Neha N. Patil
PY  - 2025
DA  - 2025/10/23
TI  - Bioaugmentation of Soil Artificially Polluted with Acetaminophen and Hydroxychloroquine
BT  - Proceedings of the International Conference on Sustainable Science and Technology for Tomorrow (SciTech 2024)
PB  - Atlantis Press
SP  - 117
EP  - 126
SN  - 3091-4442
UR  - https://doi.org/10.2991/978-94-6463-876-9_11
DO  - 10.2991/978-94-6463-876-9_11
ID  - Waghmode2025
ER  -