Optimization of Polyphenol Oxidase (PPO) Extraction from Elephant Grass and Legume Leaves with the Addition of Vitamin C, Vitamin E, and Curcumin to Enhance Enzyme Activity and Stability

DOI

10.2991/978-94-6463-880-6_34How to use a DOI?

Keywords

activity; enzyme; grass; legumes; PPO

Abstract

Polyphenol oxidase (PPO) is an enzyme with inhibiting biohydrogenation of lipid properties and found ubiquitously in many plant leaves. The extraction of PPO may require anti-oxidant substances for optimal concentration relevant to industrial application and nutritional enhancements for ruminal-lipid manipulation. This study investigated the enhancing effects of antioxidant addition, that is, vitamin C, vitamin E, and curcumin during PPO extraction of two grasses (Pennisetum purpureum and dwarf type-Pennisetum purpureum) and two legume leaves (Leucaena leuococephala and Glicidia sepium) on the PPO activity, yield, and stability. Using a combination of response surface methodology and factorial design, optimal extraction conditions-such as pH, temperature, and solvent type-were determined. The results showed that no single antioxidant under study exhibits enhancement of all intended properties. The addition of vitamin C significantly enhanced PPO activity, while vitamin E and curcumin contributed to improved enzyme stability. In conclusion the findings indicated that optimizing the extraction process of PPO requires the presence of additional multiple antioxidants to enhance enzyme yield and its functional from these plant sources.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Asri Nurul Huda
AU  - Hendrawan Soetanto
AU  - Akhmad Sabarudin
AU  - Aprodita Aprodita
AU  - Najmi A. Hanifah
AU  - Sayyid M. Ja’far
AU  - Robby B. Lana
AU  - Deliana Nurdianthi
AU  - Dimas Nugroho
AU  - Alfina Salsalbila
AU  - Aksauri H. Malis
PY  - 2025
DA  - 2025/10/31
TI  - Optimization of Polyphenol Oxidase (PPO) Extraction from Elephant Grass and Legume Leaves with the Addition of Vitamin C, Vitamin E, and Curcumin to Enhance Enzyme Activity and Stability
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 312
EP  - 318
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_34
DO  - 10.2991/978-94-6463-880-6_34
ID  - Huda2025
ER  -