Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)

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Quality of Gaga Chicken Spermatozoa During Freezing with Skim Milk Supplementation in Lactated Ringer’s Diluent

Authors
Muhammad Ikhsan1, Herlina Pratiwi2, Khaeruddin Khaeruddin3, Sri Wahjuningsih1, *
1Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
2Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, 65151, Indonesia
3Faculty of Agriculture, Universitas Muhammadiyah Sinjai, Sinjai, 92611, Indonesia
*Corresponding author. Email: yuning@ub.ac.id
Corresponding Author
Sri Wahjuningsih
Available Online 31 October 2025.
DOI
10.2991/978-94-6463-880-6_6How to use a DOI?
Keywords
skim milk; lactated ringer; diluent; spermatozoa; Gaga chicken
Abstract

The aim of this study was to assess the quality of Gaga chicken spermatozoa during freezing using lactated ringer diluent added with skim milk. This study used a completely randomized design (CRD) with 4 treatment levels (control, skim milk 5%, 10% and 15%) and 10 replicates. The research stages were diluted spermatozoa, packed in straw, equilibration, pre freezing, freezing and thawing. The parameters observed were plasma membrane integrity and spermatozoa DNA damage. Data were analyzed using analysis of variance (ANOVA), differences between treatments were analyzed using Duncan’s multiple range test. The results showed that there were significant differences (P<0.05) among treatments on plasma membrane integrity and DNA damage before freezing and post thawing and there were no significant differences (P>0.05) among treatments on decreased plasma membrane integrity and increased DNA damage. The use of lactated ringer supplemented with skim milk at a concentration of 5% resulted in better quality of chicken spermatozoa compared to other treatments during freezing.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
Series
Advances in Biological Sciences Research
Publication Date
31 October 2025
ISBN
978-94-6463-880-6
ISSN
2468-5747
DOI
10.2991/978-94-6463-880-6_6How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Muhammad Ikhsan
AU  - Herlina Pratiwi
AU  - Khaeruddin Khaeruddin
AU  - Sri Wahjuningsih
PY  - 2025
DA  - 2025/10/31
TI  - Quality of Gaga Chicken Spermatozoa During Freezing with Skim Milk Supplementation in Lactated Ringer’s Diluent
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 47
EP  - 56
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_6
DO  - 10.2991/978-94-6463-880-6_6
ID  - Ikhsan2025
ER  -