Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)

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Ultrastructure Analysis of Gaga Chicken Spermatozoa after Freezing with Coenzyme Q10 Supplementation in the Diluent

Authors
Sri Wahjuningsih1, *, Khaeruddin Khaeruddin2, Herlina Pratiwi3, Ani Atul Arif1
1Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
2Faculty of Agriculture, Universitas Muhammadiyah Sinjai, Sinjai, 92611, Indonesia
3Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, 65151, Indonesia
*Corresponding author. Email: yuning@ub.ac.id
Corresponding Author
Sri Wahjuningsih
Available Online 31 October 2025.
DOI
10.2991/978-94-6463-880-6_7How to use a DOI?
Keywords
Coenzyme Q10; Freezing; Gaga chicken; Sperm; Ultrastructure
Abstract

The purpose of this study was to analyze the ultrastructure of Gaga chicken spermatozoa after freezing-thawing with Coenzyme Q10 (CoQ10) supplementation in egg yolk lactate ringer diluent. The 3 Gaga chickens used were 10 months old, semen was collected using massage technique, diluted with egg yolk lactate ringer (EOL) with 2 treatments, namely EOL without CoQ10 and EOL with 300 μM CoQ10 supplementation. The diluted semen was packed in straws, equilibrated for 2 hours, pre-freezing for 10 minutes, freezing for 24 hours and thawing. Fresh semen and post-thawing semen were prepared, coated and observed using Field Emission Scanning Electron Microscope (FESEM). The results of the study were observed descriptively. The results of the study showed that fresh semen had intact spermatozoa ultrastructure conditions in the head, middle and tail sections, while post-thawing semen experienced damage to the head, middle and tail sections with control treatment and no damage to spermatozoa occurred with CoQ10 treatment. The conclusion of this study is that Coenzyme Q10 supplementation in egg yolk lactate ringer diluent is able to maintain intact ultrastructure in Gaga chicken spermatozoa.

Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
Series
Advances in Biological Sciences Research
Publication Date
31 October 2025
ISBN
978-94-6463-880-6
ISSN
2468-5747
DOI
10.2991/978-94-6463-880-6_7How to use a DOI?
Copyright
© 2025 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Sri Wahjuningsih
AU  - Khaeruddin Khaeruddin
AU  - Herlina Pratiwi
AU  - Ani Atul Arif
PY  - 2025
DA  - 2025/10/31
TI  - Ultrastructure Analysis of Gaga Chicken Spermatozoa after Freezing with Coenzyme Q10 Supplementation in the Diluent
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 57
EP  - 64
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_7
DO  - 10.2991/978-94-6463-880-6_7
ID  - Wahjuningsih2025
ER  -