Analysis Variation of Growth Hormone Receptor (GHR) on the Z Chromosome in Maron Chicken

Aan Setyo Nugroho; Galih Pambuko; Hasan Haidar Aqil; Rahayu Kusumaningrum; Lanjar Sumarno; Luthfi Adya Pradista; Nuzul Widyas; Adi Ratriyanto; Sigit Prastowo

doi:10.2991/978-94-6463-880-6_8

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Analysis Variation of Growth Hormone Receptor (GHR) on the Z Chromosome in Maron Chicken

Authors

Aan Setyo Nugroho¹, Galih Pambuko¹^{, 2}, Hasan Haidar Aqil¹, Rahayu Kusumaningrum³, Lanjar Sumarno⁴, Luthfi Adya Pradista¹^{, 5}, Nuzul Widyas¹, Adi Ratriyanto¹^{, 2}, Sigit Prastowo¹^{, 2}^{, *}

¹Department of Animal Science, Faculty of Animal Science, Universitas Sebelas Maret, Surakarta, Indonesia

²Master Program of Animal Science, Faculty of Animal Science, Universitas Sebelas Maret, Surakarta, Indonesia

³Doctoral Program of Agricultural Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia

⁴Research Center for Agroindustry, National Research and Innovation Agency (Badan Riset dan Inovasi Nasional), KST Soekarno Cibinong, West Java, Indonesia

⁵Vocational Program of Animal Science, Vocational School, Universitas Sebelas Maret, Surakarta, Indonesia

^*Corresponding author. Email: prastowo@staff.uns.ac.id

Corresponding Author

Sigit Prastowo

Available Online 31 October 2025.

DOI: 10.2991/978-94-6463-880-6_8 How to use a DOI?
Keywords: association; genetic variation; GHR gene; native chicken; polymorphism
Abstract: The growth hormone receptor (GHR) gene plays an important role in regulating growth as a receptor for the growth hormone (GH) gene. This study aims to analyze the variation of the GHR|Eco72I gene and its association with body size in the Maron Chicken elders. Ninety DNA samples were extracted from the blood of Kedu, Arab, and Lingnan Chickens from BBPTT Maron Chicken, Temanggung, Central Java. Amplification of the GHR|Eco72I gene in intron 5 using the PCR-RFLP method with a product size of 326 bp. The genotype variations found in the GHR|Eco72I gene were AA and AG with two types of alleles: A and G. The analysis showed that the AA genotype frequency was the highest in all populations. The highest allele frequency was the A allele (0.99), and the lowest was the G allele (0.01). The populations of Kedu, Arab, and Lingnan Chickens were in Hardy-Weinberg equilibrium. The observed heterozygosity value (Ho) in Kedu Chicken is higher than the expected heterozygosity value (He). The GHR|Eco72I polymorphism is not significantly associated with body weight, chest length, chest width, wingspan, shank length, or shank diameter. The study concludes that the GHR|Eco72I gene is polymorphic in the Kedu Chicken population but monomorphic in Arab and Lingnan Chickens.
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title: Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
Series: Advances in Biological Sciences Research
Publication Date: 31 October 2025
ISBN: 978-94-6463-880-6
ISSN: 2468-5747
DOI: 10.2991/978-94-6463-880-6_8 How to use a DOI?
Open Access: Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

ris enw bib

TY  - CONF
AU  - Aan Setyo Nugroho
AU  - Galih Pambuko
AU  - Hasan Haidar Aqil
AU  - Rahayu Kusumaningrum
AU  - Lanjar Sumarno
AU  - Luthfi Adya Pradista
AU  - Nuzul Widyas
AU  - Adi Ratriyanto
AU  - Sigit Prastowo
PY  - 2025
DA  - 2025/10/31
TI  - Analysis Variation of Growth Hormone Receptor (GHR) on the Z Chromosome in Maron Chicken
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 65
EP  - 75
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_8
DO  - 10.2991/978-94-6463-880-6_8
ID  - Nugroho2025
ER  -

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