Comparative Effectiveness of Thawing Temperature and Duration on the Quality of Cryopreserved River Buffalo Semen

DOI

10.2991/978-94-6463-880-6_27How to use a DOI?

Keywords

buffalo semen; thawing; semen quality

Abstract

This study investigates the effect of varying thawing duration and temperature on the quality of cryopreserved river buffalo semen. A randomized group design with four treatments and ten replications was used, followed by Analysis of Variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) for significant differences. The thawing treatments included 30 seconds at 37˚C (T0) according to SNI, and 30 seconds (T1), 45 seconds (T2), and 60 seconds (T3) using water at 28˚C. The observed variables were motility, concentration, total motile spermatozoa, viability, abnormality, and plasma membrane integrity. The results revealed that thawing duration and temperature significantly affected motility, total motile spermatozoa, viability, and plasma membrane integrity (P < 0.01), while abnormality and concentration were not significantly influenced (P > 0.05). The best quality of frozen buffalo semen was obtained using water at 28˚C for 60 seconds, with individual motility at 43.50%, sperm concentration of 29.31 million/straw, total motile spermatozoa at 12.88 million/straw, viability of 81.64%, abnormalities at 16.31%, and membrane integrity of 75.40%. These findings suggest that while the SNI standard of thawing at 37˚C remains optimal, thawing at 28˚C for 60 seconds offers a practical alternative under field conditions.

Copyright

© 2025 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Download article (PDF)

TY  - CONF
AU  - Naurah Nazifah
AU  - Nurul Isnaini
PY  - 2025
DA  - 2025/10/31
TI  - Comparative Effectiveness of Thawing Temperature and Duration on the Quality of Cryopreserved River Buffalo Semen
BT  - Proceedings of the 11th International Conference of Innovation in Animal Science (ICIAS 2024)
PB  - Atlantis Press
SP  - 238
EP  - 249
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-880-6_27
DO  - 10.2991/978-94-6463-880-6_27
ID  - Nazifah2025
ER  -